ELISA Kit for Indole 3 Acetic Acid (IAA)
| Code | Size | Price |
|---|
| CEA737Ge-24T | 24T | £242.00 |
Quantity:
| CEA737Ge-48T | 48T | £401.00 |
Quantity:
| CEA737Ge-96T | 96T | £560.00 |
Quantity:
| CEA737Ge-96T*5 | 96T*5 | £2,413.00 |
Quantity:
| CEA737Ge-96T*10 | 96T*10 | £4,530.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Species Independent
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Further Information
Alternative Names:
IA-A; Indolylacetic Acid; Indoleacetic Acid; Heteroauxin
Assay length:
2h
Assay Procedure Summary:
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Detection range:
2.47-200ng/mL
Format:
48T, 96T, 96T?5, 96T?10, 96T?100
Item Name:
Indole 3 Acetic Acid
Method:
Competitive Inhibition
Precision:
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Indole 3 Acetic Acid (IAA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Indole 3 Acetic Acid (IAA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Indole 3 Acetic Acid (IAA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Research Area:
Signal transduction;Hormone metabolism;
Sample type:
Tissue or cell culture supernates
Sensitivity:
The minimum detectable dose of this kit is typically less than 0.89ng/mL
Specificity:
This assay has high sensitivity and excellent specificity for detection of Indole 3 Acetic Acid (IAA).
No significant cross-reactivity or interference between Indole 3 Acetic Acid (IAA) and analogues was observed.
No significant cross-reactivity or interference between Indole 3 Acetic Acid (IAA) and analogues was observed.
Stability:
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Indole 3 Acetic Acid (IAA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Indole 3 Acetic Acid (IAA) and unlabeled Indole 3 Acetic Acid (IAA) (Standards or samples) with the pre-coated antibody specific to Indole 3 Acetic Acid (IAA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Indole 3 Acetic Acid (IAA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Indole 3 Acetic Acid (IAA) in the sample.
References
http://www.ncbi.nlm.nih.gov/pubmed/21570105; http://link.springer.com/article/10.1007/s00374-014-0978-8; https://doi.org/10.1016/j.plantsci.2019.02.001; https://www_ncbi.gg363.site/pubmed/31484323;