CLIA Kit for Transforming Growth Factor Alpha (TGFa)
| Code | Size | Price |
|---|
| SCA123Hu-24T | 24T | £254.00 |
Quantity:
| SCA123Hu-48T | 48T | £422.00 |
Quantity:
| SCA123Hu-96T | 96T | £589.00 |
Quantity:
| SCA123Hu-96T*5 | 96T*5 | £2,544.00 |
Quantity:
| SCA123Hu-96T*10 | 96T*10 | £4,779.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Application: Chemiluminescent Immunoassay (CLIA)
Further Information
Alternative Names:
ETGF; TGF-A; Protransforming Growth Factor alpha; EGF-Like TGF
Assay length:
2h, 40min
Assay Procedure Summary:
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
Detection range:
31.25-2000pg/mL
Format:
48T, 96T, 96T?5, 96T?10, 96T?100
Item Name:
Transforming Growth Factor Alpha
Method:
Double-antibody Sandwich
Precision:
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Alpha (TGFa) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Alpha (TGFa) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Alpha (TGFa) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Research Area:
Cytokine;Tumor immunity;Infection immunity;
Sample type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity:
The minimum detectable dose of this kit is typically less than 0.94pg/mL
Specificity:
This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Alpha (TGFa).
No significant cross-reactivity or interference between Transforming Growth Factor Alpha (TGFa) and analogues was observed.
No significant cross-reactivity or interference between Transforming Growth Factor Alpha (TGFa) and analogues was observed.
Stability:
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Test Principle:
The microplate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Alpha (TGFa). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Alpha (TGFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Transforming Growth Factor Alpha (TGFa) level in the sample or standard.;
Uniport ID:
P01135