TIP60 (human) (rec.) (His) (highly active)
| Code | Size | Price |
|---|
| AG-40T-0019-C002 | 2 ug | £187.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Shipping:
Dry Ice
Storage:
-20°C
Documents
Further Information
Alternate Names/Synonyms:
Histone Acetyltransferase HTATIP; Histone Acetyltransferase KAT5; Lysine Acetyltransferase 5; 60 kDa Tat-interactive Protein
Biological Activity:
100-200ng are required for standard HAT assay.
Concentration:
Lot dependent (0.2-0.5mg/ml)
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50mM imidazole and 10% glycerol.
Handling Advice:
After opening, prepare aliquots and store at -80°C.Avoid freeze/thaw cycles.
Long Description:
Protein. Human TIP60 is fused to a His-tag. Source: Sf21 cells. Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50mM imidazole and 10% glycerol. Purity: >95% (SDS-PAGE). TIP60 is the catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex is required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. TIP60 directly acetylates and activates ataxia-telangiectasia mutated (ATM). In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation.
NCBI, Uniprot Number:
Q92993
Package Type:
Plastic Vial
Product Description:
TIP60 is the catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex is required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. TIP60 directly acetylates and activates ataxia-telangiectasia mutated (ATM). In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation.
Purity:
>95% (SDS-PAGE)
Sequence:
Human TIP60 is fused to a His-tag.
Source / Host:
Sf21 cells
TAGs:
His
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 6 months after receipt when stored at -80°C.
References
Functional relevance of novel p300-mediated lysine 314 and 315 acetylation of RelA/p65: C. Buerki, et al.; Nucleic Acids Res. 36, 1665 (2008) | Oridonin, a novel lysine acetyltransferases inhibitor, inhibits proliferation and induces apoptosis in gastric cancer cells through p53- and caspase-3-mediated mechanisms: M. Shi, et al.; Oncotarget 7, 22623 (2016)