NMNAT1 (human) (rec.) (His) (highly active)
| Code | Size | Price |
|---|
| AG-40T-0092-C010 | 10 ug | £180.00 |
Quantity:
| AG-40T-0092-C050 | 50 ug | £310.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Shipping:
Blue Ice
Storage:
-20°C
Documents
Further Information
Alternate Names/Synonyms:
Nicotinamide Mononucleotide Adenylyltransferase 1; NMN Adenylyltransferase 1; NaMN Adenylyltransferase 1; EC 2.7.7.1
Biological Activity:
>5U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1µmol of NAD+ per min.
Concentration:
~1mg/ml
EClass:
32160000
Endotoxin:
N/A
Form (Short):
liquid
Formulation:
Liquid. In 50mM sodium phosphate, pH8.0, containing 300mM sodium chloride, 2mM DTT and 10% glycerol.
Handling Advice:
After opening, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.
Long Description:
Protein. Human full-length NMNAT1 (aa 1-279) is fused at the N-terminus to a His-tag. Source: E. coli. Endotoxin content: N/A. Liquid. In 50mM sodium phosphate, pH8.0, containing 300mM sodium chloride, 2mM DTT and 10% glycerol. Purity: >95% (SDS-PAGE). Nuclear NMNAT isoform. Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. It can also use the deamidated form of nicotinic acid mononucleotide (NAMN) as substrate with the same efficiency. Interacts with PARP-1/ARTD1. Protects against axonal degeneration following mechanical or toxic insults. Widely expressed.
Molecular Weight:
~33.3kDa (monomer)
NCBI, Uniprot Number:
Q9HAN9
Package Type:
Plastic Vial
Product Description:
Nuclear NMNAT isoform. Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. It can also use the deamidated form of nicotinic acid mononucleotide (NAMN) as substrate with the same efficiency. Interacts with PARP-1/ARTD1. Protects against axonal degeneration following mechanical or toxic insults. Widely expressed.
Purity:
>95% (SDS-PAGE)
Sequence:
Human full-length NMNAT1 (aa 1-279) is fused at the N-terminus to a His-tag.
Source / Host:
E. coli
TAGs:
His
Transportation:
Non-hazardous
UNSPSC Category:
Other Enzymes
UNSPSC Number:
12352204
Use & Stability:
Working aliquots are stable for up to 3 months when stored at -20°C.
References
Molecular cloning, chromosomal localization, tissue mRNA levels, bacterial expression, and enzymatic properties of human NMN adenylyltransferase: M. Emanuelli, et al.; J. Biol. Chem. 276, 406 (2001) | Characterization of recombinant human nicotinamide mononucleotide adenylyl transferase (NMNAT), a nuclear enzyme essential for NAD synthesis: M. Schweiger, et al.; FEBS Lett. 492, 95 (2001) | Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms: F. Berger, et al.; J. Biol. Chem. 280, 36334 (2005) | The NMN/NaMN adenylyltransferase (NMNAT) protein family: C. Lau, et al.; Front. Biosci. 14, 410 (2009) | Isoform-specific targeting and interaction domains in human nicotinamide mononucleotide adenylyltransferases: C. Lau, et al.; J. Biol. Chem. 285, 18868 (2010)