CD275 [B7-H2] (human):Fc (human) (rec.) (non-lytic)
| Code | Size | Price |
|---|
| CHI-HF-220B7H2-C100 | 100 ug | £395.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Target Species: Human
Shipping:
BI
Storage:
-20°C
Further Information
Alternate Names/Synonyms:
B7-H2; ICOS Ligand; B7-like Protein Gl50; B7RP-1
Biological Activity:
Measured by its ability to stimulate human T cell proliferation in the presence of anti-CD3.
EClass:
32160000
Endotoxin:
<0.06EU/µg protein (LAL test; Lonza).
Form (Short):
solid
Formulation:
Lyophilized from 0.2µm-filtered solution in PBS.
Handling Advice:
Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.
Long Description:
Protein. The extracellular domain of human CD275 [B7-H2] (aa 1-256) is fused to the N-terminus of the Fc region of a mutant human IgG1. Source: CHO cells. Endotoxin content: <0.06EU/µg protein (LAL test; Lonza). Lyophilized from 0.2µm-filtered solution in PBS. Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgammaR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC). Purity: >98% (SDS-PAGE). Human B7-H2 is a member of the growing B7 family of immune costimulatory proteins. Other family members include B7-1, B7-2, B7-H1 (PD-L1), PD-L2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
NCBI, Uniprot Number:
NP_056074.1
Package Type:
Plastic Vial
Product Description:
Human B7-H2 is a member of the growing B7 family of immune costimulatory proteins. Other family members include B7-1, B7-2, B7-H1 (PD-L1), PD-L2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
Purity:
>98% (SDS-PAGE)
Sequence:
The extracellular domain of human CD275 [B7-H2] (aa 1-256) is fused to the N-terminus of the Fc region of a mutant human IgG1.
Source / Host:
CHO cells
Specificity:
Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgammaR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).
TAGs:
Fc
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.