anti-Profilin pAb (IG-706)
| Code | Size | Price |
|---|
| AG-25T-0117-C010 | 10 ug | £300.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Bovine (Cattle)
- Human
- Mouse
- Porcine (pig)
Applications:
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
-20°C
Storage:
-20°C
Documents
Further Information
Alternate Names/Synonyms:
PFN1
Concentration:
100µg/ml
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In PBS containing 1mg/ml BSA and 0.02% sodium azide.
Handling Advice:
Avoid freeze/thaw cycles.
Immunogen:
Human full-length profilin.
Long Description:
Polyclonal Antibody. Recognizes human, mouse, rat, pig, bovine and marsupial profilin. Source: Rabbit. Applications: ICC, IHC, WB. Liquid. In PBS containing 1mg/ml BSA and 0.02% sodium azide. Profilin (PFN1) is a ubiquitous small (12-15kDa) phosphoinositide and poly-L-proline binding protein that plays a role in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels also in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes. Profilin binding to PIP2 interferes with PIP2 hydrolysis by soluble phospholipase C-gamma, an inhibition that can be overcome by tyrosine phosphorylation of PLC-gamma. Besides actin monomer sequestration and stimulation of actin nucleotide exchange, profilin can also promote cellular actin filament growth. Profilin is involved in the actin dependent intracellular motility of cytopathogenic bacteria, the regulation of cell adhesion and possibly also in linking the actin cytoskeleton and endocytosis. Profilin has been found to associate with defined complexes containing proteins such as Arp2/3 or the Rho/Rac pathways constituents ROCK-II and HEM2/NAP1. Defects in PFN1 are the cause of amyotrophic lateral sclerosis 18 (ALS18).
NCBI, Uniprot Number:
P07737
Package Type:
Plastic Vial
Product Description:
Profilin (PFN1) is a ubiquitous small (12-15kDa) phosphoinositide and poly-L-proline binding protein that plays a role in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels also in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes. Profilin binding to PIP2 interferes with PIP2 hydrolysis by soluble phospholipase C-gamma, an inhibition that can be overcome by tyrosine phosphorylation of PLC-gamma. Besides actin monomer sequestration and stimulation of actin nucleotide exchange, profilin can also promote cellular actin filament growth. Profilin is involved in the actin dependent intracellular motility of cytopathogenic bacteria, the regulation of cell adhesion and possibly also in linking the actin cytoskeleton and endocytosis. Profilin has been found to associate with defined complexes containing proteins such as Arp2/3 or the Rho/Rac pathways constituents ROCK-II and HEM2/NAP1. Defects in PFN1 are the cause of amyotrophic lateral sclerosis 18 (ALS18).
Purity:
Antigen affinity purified.
Source / Host:
Rabbit
Specificity:
Recognizes human, mouse, rat, pig, bovine and marsupial profilin.
Transportation:
Non-hazardous
UNSPSC Category:
Primary Antibodies
UNSPSC Number:
12352203
Use & Stability:
Stable for at least 1 year after receipt when stored at -20°C.
References
A role for polyproline motifs in the spinal muscular atrophy protein SMN. Profilins bind to and colocalize with smn in nuclear gems: T. Giesemann, et al.; J. Biol. Chem. 274, 37908 (1999) | Coordinate induction of the actin cytoskeletal regulatory proteins gelsolin, vasodilator-stimulated phosphoprotein, and profilin during capillary morphogenesis in vitro: R. Salazar, et al.; Exp. Cell. Res. 249, 22 (1999) | Megakaryocyte hyperplasia and enhanced agonist-induced platelet activation in vasodilator-stimulated phosphoprotein knockout mice: W. Hauser, et al.; PNAS 96, 8120 (1999) | Drebrin particles: components in the ensemble of proteins regulating actin dynamics of lamellipodia and filopodia: W.K. Peitsch, et al.; Eur. J. Cell Biol. 80, 567 (2001) | Mutant actins that stabilise F-actin use distinct mechanisms to activate the SRF coactivator MAL: G. Posern, et al.; EMBO J. 23, 3973 (2004) | Profilin and actin-related proteins regulate microfilament dynamics during early mammalian embryogenesis: V.Y. Rawe, et al.; Hum. Reprod. 21, 1143 (2006) | Exploring the Cytoskeleton During Intracytoplasmic Sperm Injection in Humans: V.Y. Rawe & H. Chemes; Microinjection 518, 189 (2009) | Decellularized Matrix from Tumorigenic Human Mesenchymal Stem Cells Promotes Neovascularization with Galectin-1 Dependent Endothelial Interaction: J.S. Burns, et al.; PLoS ONE 6, e21888 (2011) | Nikolova-Krstevski, V. et al. Nesprin-1 and actin contribute to nuclear and cytoskeletal defects in lamin A/C-deficient cardiomyopathy. J. Mol. Cell. Cardiol. 50, 479 (2011)