TDO (Anopheles gambiae) (rec.) (His)

TPH2; TRPO; Tryptophan 2,3-dioxygenase; Tryptophan Oxygenase; Tryptophan Pyrrolase; Tryptophanase

Specific activity towards L-Trp: 87.0µmol/hr per mg. TDO catalyzes the oxidative opening of the L-tryptophan indole moiety to yield N-formylkynurenine (NFK).

0.5mg/ml (Lot specific)

32160000

liquid

Liquid. In 50mM sodium phosphate pH 8.0, containing 0.15M sodium chloride, 0.2M imidazole and 10% glycerol.

After opening, prepare aliquots and store at -80°C.Avoid freeze/thaw cycles.

Protein. Full-length TDO (Anopheles gambiae) is fused at the N-terminus to a His-tag. Source: E. coli. Endotoxin content: N/A. Liquid. In 50mM sodium phosphate pH 8.0, containing 0.15M sodium chloride, 0.2M imidazole and 10% glycerol. Purity: >98% (SDS-PAGE). Tryptophan 2,3-dioxygenase (TDO) is a heme-containing dioxygenase catalyzing the addition of molecular oxygen across the 2,3-double bond of the indole ring of tryptophan to form N-formylkynurenine (NFK). In Anopheles gambiae, TDO is the only enzyme able to catalyze the first and rate-limiting step in L-Trp catabolism through the kynurenine pathway. Tryptophan is an essential amino acid for protein synthesis and also the precursor for production of a number of neurotransmitters, such as serotonin and melatonin; in mosquitoes, the kynurenine pathway is essential for eye pigmentation. Conceivably, the tryptophan-using pathways should be regulated in a coordinated manner in mosquitoes as well as in other species and TDO activation/inactivation processes could play an essential role in these phenomena.

~47.9kDa

O77457

Plastic Vial

Tryptophan 2,3-dioxygenase (TDO) is a heme-containing dioxygenase catalyzing the addition of molecular oxygen across the 2,3-double bond of the indole ring of tryptophan to form N-formylkynurenine (NFK). In Anopheles gambiae, TDO is the only enzyme able to catalyze the first and rate-limiting step in L-Trp catabolism through the kynurenine pathway. Tryptophan is an essential amino acid for protein synthesis and also the precursor for production of a number of neurotransmitters, such as serotonin and melatonin; in mosquitoes, the kynurenine pathway is essential for eye pigmentation. Conceivably, the tryptophan-using pathways should be regulated in a coordinated manner in mosquitoes as well as in other species and TDO activation/inactivation processes could play an essential role in these phenomena.

>98% (SDS-PAGE)

Full-length TDO (Anopheles gambiae) is fused at the N-terminus to a His-tag.

Synthetic.

His

Non-hazardous

Other Enzymes

12352204

Stable for at least 6 months after receipt when stored at -80°C.