NAD Kinase (B. subtilis) (rec.)
| Code | Size | Price |
|---|
| AG-40T-0106-C050 | 50 ug | £317.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Antibody Clonality: Enzyme
Regulatory Status: RUO
Shipping:
Dry Ice
Storage:
-80°C
Documents
Further Information
Alternate Names/Synonyms:
Probable Inorganic Polyphosphate/ATP-NAD Kinase; Poly(P)/ATP NAD Kinase; ppnK; yjbN; EC 2.7.1.23
Biological Activity:
~2.6 U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1µmol nicotinamide adenine dinucleotide phosphate (NADP) per min.
Concentration:
Lot specific
EClass:
32160000
Form (Short):
liquid
Formulation:
Liquid. In 50mM TRIS-HCl, pH 7.0 containing 150mM sodium chloride, 1mM EDTA and 1mM dithiothreitol.
Handling Advice:
After opening, prepare aliquots and store at -80°C.Avoid freeze/thaw cycles.
Long Description:
Protein. Full-length NAD kinase (Bacillus subtilis). Source: E. coli. Endotoxin content: N/A. Liquid. In 50mM TRIS-HCl, pH 7.0 containing 150mM sodium chloride, 1mM EDTA and 1mM dithiothreitol. Purity: >98% (SDS-PAGE). NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from Bacillus subtilis is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP+ by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus. Catalytic activity: ATP + NAD+ = ADP + NADP+
Molecular Weight:
~30.0kDa
NCBI, Uniprot Number:
O31612
Package Type:
Plastic Vial
Product Description:
NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from Bacillus subtilis is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP+ by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus. Catalytic activity: ATP + NAD+ = ADP + NADP+
Purity:
>98% (SDS-PAGE)
Sequence:
Full-length NAD kinase (Bacillus subtilis).
Source / Host:
E. coli
TAGs:
no TAG
Transportation:
Non-hazardous
UNSPSC Category:
Other Enzymes
UNSPSC Number:
12352204
Use & Stability:
Stable for at least 6 months after receipt when stored at -80°C.
References
Allosteric regulation of Bacillus subtilis NAD kinase by quinolinic acid: S. Garavaglia, et al.; J. Bacteriol. 185, 4844 (2003)