NAD Kinase (M. tuberculosis) (His)
| Code | Size | Price |
|---|
| AG-40T-0107-C050 | 50 ug | £317.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Antibody Clonality: Enzyme
Regulatory Status: RUO
Shipping:
Dry Ice
Storage:
-80°C
Documents
Further Information
Alternate Names/Synonyms:
Probable Inorganic Polyphosphate/ATP-NAD kinase; Poly(P)/ATP NAD Kinase 1; ppnK1; EC 2.7.1.23
Biological Activity:
~1.2 U/mg protein. (Substrate: NAD (+ ATP))
~3.8 U/mg protein. (Substrate: NAD (+ poly(P))
One unit is defined as the amount of enzyme that synthesizes 1µmol nicotinamide adenine dinucleotide phosphate (NADP) per min.
Concentration:
0.5mg/ml (Lot specific)
EClass:
32160000
Endotoxin:
N/A
Form (Short):
liquid
Formulation:
Liquid. In 10mM TRIS at pH 8.0, containing 1mM MgCl2, 0.5mM EDTA, 1mM DTT and 1mM PMSF.
Handling Advice:
After opening, prepare aliquots and store at -80°C.Avoid freeze/thaw cycles.
Long Description:
Protein. NAD kinase (Mycobacterium tuberculosis) is fused at the N-terminus to a His-tag. Source: E. coli. Endotoxin content: N/A. Liquid. In 10mM TRIS at pH 8.0, containing 1mM MgCl2, 0.5mM EDTA, 1mM DTT and 1mM PMSF. Purity: >98% (SDS-PAGE). NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from Mycobacterium tuberculosis is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP+ by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus. Was identified as a high-confidence drug target. Catalytic activity: ATP + NAD+ = ADP + NADP+
Molecular Weight:
~33.0kDa
NCBI, Uniprot Number:
P0A5S6
Package Type:
Plastic Vial
Product Description:
NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from Mycobacterium tuberculosis is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP+ by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus. Was identified as a high-confidence drug target. Catalytic activity: ATP + NAD+ = ADP + NADP+
Purity:
>98% (SDS-PAGE)
Sequence:
NAD kinase (Mycobacterium tuberculosis) is fused at the N-terminus to a His-tag.
Source / Host:
E. coli
TAGs:
His
Transportation:
Non-hazardous
UNSPSC Category:
Other Enzymes
UNSPSC Number:
12352204
Use & Stability:
Stable for at least 6 months after receipt when stored at -80°C.
References
Characterization of Mycobacterium tuberculosis NAD Kinase: Functional Analysis of the Full-Length Enzyme by Site-Directed Mutagenesis: N. Raffaelli, et al.; Biochemistry 43, 7610 (2004) | A Novel Fold Revealed by Mycobacterium tuberculosis NAD Kinase, a Key Allosteric Enzyme in NADP Biosynthesis: S. Garavaglia, et al.; J. Biol. Chem. 279, 40980 (2004)