NAD Synthetase (M. tuberculosis) (rec.) (His)

NADE; Glutamine-dependent NAD(+) Synthetase; EC 6.3.5.1

~0.1 U/mg protein. (Ammonium or glutamine as nitrogen source). One unit is defined as the amount of enzyme that synthesizes 1µmol NAD per min.

0.5mg/ml (Lot specific)

32160000

liquid

Liquid. In 20mM TRIS-HCl, pH 7.8.

After opening, prepare aliquots and store at -80°C.Avoid freeze/thaw cycles.

Protein. NAD synthetase (Mycobacterium tuberculosis) (with mutation S444A) is fused at the N-terminus to a His-tag. Source: E. coli. Endotoxin content: N/A. Liquid. In 20mM TRIS-HCl, pH 7.8. Purity: >98% (SDS-PAGE). NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It can use both glutamine or ammonia as a nitrogen source. Catalytic activity: ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate

~75.0kDa

P0A5L6

Plastic Vial

NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It can use both glutamine or ammonia as a nitrogen source. Catalytic activity: ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate

>98% (SDS-PAGE)

NAD synthetase (Mycobacterium tuberculosis) (with mutation S444A) is fused at the N-terminus to a His-tag.

E. coli

His

Non-hazardous

Other Enzymes

12352204

Stable for at least 6 months after receipt when stored at -80°C.