DC-SIGN Monoclonal Antibody

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ProSci
Product Code: PSI-PM-2347
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-PM-2347-0.02mg0.02mg£150.00
Quantity:
PSI-PM-2347-0.1mg0.1mg£515.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Clonality: Monoclonal
Antibody Clone: 5D7
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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<strong>Figure 1 WB Validation of DC-SIGN in Human Lymph Node</strong><br> Loading: 10 ug of lysate per lane. Antibodies: DC-SIGN PM-2347, 1ug/ml, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Lane 1: 1 ug/mL Lane 2: 2 ug/mL
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Immunohistochemistry of DC-SIGN in human lymph node tissue with DC-SIGN antibody at 5 μg/mL.
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<strong>Figure 1 WB Validation of DC-SIGN in Human Lymph Node</strong><br> Loading: 10 ug of lysate per lane. Antibodies: DC-SIGN PM-2347, 1ug/ml, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Lane 1: 1 ug/mL Lane 2: 2 ug/mL
Immunohistochemistry of DC-SIGN in human lymph node tissue with DC-SIGN antibody at 5 μg/mL.

Documents

Further Information

Additional Names:
DC-SIGN Antibody [5D7] : CDSIGN, CLEC4L, DC-SIGN, DC-SIGN1
Application Note:
DC-SIGN antibody can be used for detection of DC-SIGN in Western Blot at 1 μg/mL and in immunohistochemistry at 5 - 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
DC-SIGN Monoclonal Antibody: Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes, where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-SIGN. ICAM-3 is the natural ligand for DC-SIGN. A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-SIGN2) was identified recently. DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR. The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum.
Background References:
  • Geijtenbeek TB, Kwon DS, Torensma R, et al. DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells. Cell 2000; 100:587-97.
  • Pohlmann S, Baribaud F, Lee B, et al. DC-SIGN interactions with human immunodeficiency virus type 1 and 2 and simian immunodeficiency virus. J. Virol. 2001; 75:4664-72.
  • Geijtenbeek TB, Torensma R, van Vliet SJ, et al. Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses. Cell 2000; 100:575-85.
  • Soilleux EJ, Barten R, Trowsdale J. DC-SIGN; a related gene, DC-SIGNR; and CD23 form a cluster on 19p13. J. Immunol. 2000; 165:2937-42.
Buffer:
DC-SIGN Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A recombinant His-tagged protein fragment corresponding to the extracellular region of human DC-SIGN.
NCBI Gene ID #:
30835
NCBI Official Name:
CD209 molecule
NCBI Official Symbol:
CD209
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 44 kDa

Observed: 47 kDa
Protein Accession #:
Q9NNX6
Protein GI Number:
46396012
Purification:
DC-SIGN Monoclonal Antibody is Protein A purified.
Research Area:
Infectious Disease
Swissprot #:
Q9NNX6
User NOte:
Optimal dilutions for each application to be determined by the researcher.