PUMA Monoclonal Antibody

Product Code: PSI-PM-4529

Supplier: ProSci

Code	Size	Price

PSI-PM-4529-0.02mg

0.02mg

£150.00

Quantity:

PSI-PM-4529-0.1mg

0.1mg

£515.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Mouse

Antibody Isotype: IgG1

Antibody Clonality: Monoclonal

Antibody Clone: 10D4G7

Regulatory Status: RUO

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Western Blot (WB)

Images

1 / 1

Western blot analysis of PUMA expression in K562 cell lysate with PUMA antibody at (A) 2.5 and (B) 5 μg/mL.

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Documents

Further Information

Additional Names:

PUMA Antibody [10D4G7] : JFY1, PUMA, JFY-1

Application Note:

PUMA antibody can be used for detection of PUMA by Western blot at 2.5 to 5 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Background:

PUMA Monoclonal Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMAα and PUMAβ. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Background References:

Nakano K, Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell. 2001; 7:683-94.
Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell. 2001; 7:673-82.
Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc Natl Acad Sci U S A. 2001; 98:11318-23.

Buffer:

PUMA Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration:

1 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Immunogen:

Recombinant protein corresponding to amino acids 76 - 170 of human PUMA-alpha.

NCBI Gene ID #:

27113

NCBI Official Name:

BCL2 binding component 3

NCBI Official Symbol:

BBC3

NCBI Organism:

Homo sapiens

Physical State:

Liquid

Protein Accession #:

Q9BXH1

Protein GI Number:

56748610

Purification:

PUMA Monoclonal Antibody is Protein A purified.

Research Area:

Apoptosis

Swissprot #:

Q9BXH1

User NOte:

Optimal dilutions for each application to be determined by the researcher.

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