Bim Monoclonal Antibody

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ProSci
Product Code: PSI-PM-4819
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-PM-4819-0.02mg0.02mg£150.00
Quantity:
PSI-PM-4819-0.1mg0.1mg£515.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 1C2C8
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of (A) 5 and (B) 25 ng of Bim recombinant protein with Bim antibody at 1 μg/mL.
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Immunocytochemistry of Bim in K562 cells with Bim antibody at 10 μg/mL.
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Immunofluorescence of Bim in K562 cells with Bim antibody at 20 μg/mL.
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Western blot analysis of (A) 5 and (B) 25 ng of Bim recombinant protein with Bim antibody at 1 μg/mL.
Immunocytochemistry of Bim in K562 cells with Bim antibody at 10 μg/mL.
Immunofluorescence of Bim in K562 cells with Bim antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
Bim Antibody [1C2C8] : BAM, BIM, BOD
Application Note:
Bim antibody can be used for detection of Bim by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Bim Monoclonal Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. Bim is another BH3 domain containing protein which can induce apoptosis. Bim interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.
Background References:
  • O'Connor L, Strasser A, O'Reilly LA, et al. Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 1998; 17:384-395.
  • Hsu SY, Lin P, and Hsueh AJ BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members. Mol. Endocrinol. 1998; 12:1432-40.
Buffer:
Bim Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Mouse monoclonal Bim antibody was raised against a recombinant protein corresponding to amino acids 2 to 198 of human Bim.
NCBI Gene ID #:
10018
NCBI Official Name:
BCL2-like 11 (apoptosis facilitator)
NCBI Official Symbol:
BCL2L11
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_619527
Protein GI Number:
20336315
Purification:
Bim Monoclonal Antibody is immunoaffinity chromotography purified IgG.
Research Area:
Apoptosis,Cancer
Swissprot #:
O43521
User NOte:
Optimal dilutions for each application to be determined by the researcher.