MD-2 Monoclonal Antibody

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ProSci
Product Code: PSI-PM-4847
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-PM-4847-0.02mg0.02mg£150.00
Quantity:
PSI-PM-4847-0.1mg0.1mg£515.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 1A2E3
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 μg/mL.
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Immunohistochemistry of MD-2 in human spleen with MD-2 antibody at 2.5 μg/mL.
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Immunohistochemistry of MD-2 in human spleen with MD-2 Antibody at 2 μg/ml.
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Immunofluorescence of MD-2 in human spleen with MD-2 Antibody at 20 μg/ml.
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Western blot analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 μg/mL.
Immunohistochemistry of MD-2 in human spleen with MD-2 antibody at 2.5 μg/mL.
Immunohistochemistry of MD-2 in human spleen with MD-2 Antibody at 2 μg/ml.
Immunofluorescence of MD-2 in human spleen with MD-2 Antibody at 20 μg/ml.

Documents

Further Information

Additional Names:
MD-2 Antibody [1A2E3] : MD2, MD-2, ly-96, ESOP-1
Application Note:
MD-2 antibody can be used for detection of MD-2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.

Antibody validated: Western Blot in recombinnat protein; Immunohistochemistry in human samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
MD-2 Monoclonal Antibody: MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.
Background References:
  • O'Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9.
  • Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
  • Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
  • Janeway CA Jr and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
Buffer:
MD-2 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Mouse monoclonal MD-2 antibody was raised against a recombinant protein corresponding to amino acids 2 to 160 of human MD-2.
NCBI Gene ID #:
23643
NCBI Official Name:
lymphocyte antigen 96
NCBI Official Symbol:
LY96
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_056179
Protein GI Number:
223555998
Purification:
MD-2 Monoclonal Antibody is Protein A purified.
Research Area:
Innate Immunity
Swissprot #:
Q9Y6Y9
User NOte:
Optimal dilutions for each application to be determined by the researcher.