XBP-1 Monoclonal Antibody

Product Code: PSI-PM-4937

Supplier: ProSci

Code	Size	Price

PSI-PM-4937-0.02mg

0.02mg

£150.00

Quantity:

PSI-PM-4937-0.1mg

0.1mg

£515.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Mouse

Antibody Clonality: Monoclonal

Antibody Clone: 9B7E5

Regulatory Status: RUO

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Western Blot (WB)

Images

1 / 3

Western blot analysis of 100 ng of XBP-1 recombinant protein with XBP-1 antibody at 1 μg/mL.

2 / 3

Immunocytochemistry of XBP-1 in HepG2 cells with XBP-1 antibody at 2 μg/mL.

3 / 3

Immunofluorescence of XBP1 in HepG2 cells with XBP1 antibody at 2 μg/mL.

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Documents

Further Information

Additional Names:

XBP-1 Antibody [9B7E5] : XBP2, TREB5, XBP-1

Application Note:

XBP-1 antibody can be used for the detection of XBP-1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 4 μg/mL.

Antibody validated: Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Background:

XBP-1 Monoclonal Antibody: X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER). Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.

Background References:

Yoshida H, Matsui T, Yamamoto T, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1p in response to ER stress to produce a highly active transcription factor. Cell 2001; 107:881-91.
Calfon M, Zeng H, Urano F, et al. IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA. Nature 2002; 415:92-6.
Haze K, Yoshida H, Yanagi H, et al. Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic stress. Mol. Cell. Biol. 1999; 10:3787-99.
Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.

Buffer:

XBP-1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration:

1 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Immunogen:

Mouse monoclonal XBP-1 antibody was raised against recombinant protein corresponding to amino acids 2 to 160 of human XBP-1.

NCBI Gene ID #:

7494

NCBI Official Name:

X-box binding protein 1

NCBI Official Symbol:

XBP1

NCBI Organism:

Homo sapiens

Physical State:

Liquid

Protein Accession #:

BAB82982

Protein GI Number:

18148382

Purification:

XBP-1 Monoclonal Antibody is immunoaffinity chromotography purified IgG.

Research Area:

Signal Transduction

Swissprot #:

P17861

User NOte:

Optimal dilutions for each application to be determined by the researcher.

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