CD4 Monoclonal Antibody

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ProSci
Product Code: PSI-PM-5201
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-PM-5201-0.02mg0.02mg£150.00
Quantity:
PSI-PM-5201-0.1mg0.1mg£515.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 9H5A8
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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<strong>Figure 1 Western Blot Validation with Human Recombinant Protein</strong><br> Loading: 5ng (Lane 1), 25ng (Lane 2) and 100ng (Lane 3) of human CD4 recombinant protein. Antibodies: CD4 PM-5201 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Observed at around 27kD.
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<strong>Figure 2 Western Blot Validation in Human Thymus Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CD4 PM-5201 (Lane 1: 0.5 μg/mL, Lane 2: 1 μg/mL and Lane 3: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
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<strong>Figure 3 Western Blot Validation in Human Jurkat Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CD4 PM-5201 (Lane 1: 1 μg/mL, Lane 2: 2 μg/mL and Lane 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
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<strong>Figure 4 Western Blot Validation in Human, Mouse and Rat Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CD4 PM-5201 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
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<strong>Figure 5 Western Blot Validation in Human Cell Lines </strong><br> Loading: 15 μg of lysates per lane. Antibodies: CD4 PM-5201 (4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
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<strong>Figure 6 Western Blot Validation in Mouse Spleen Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CD4 PM-5201 (Lane 1: 1 μg/mL, Lane 2: 2 μg/mL and Lane 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
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<strong>Figure 7 Western Blot Validation in Rat Lung Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CD4 PM-5201 (Lane 1: 0.5 μg/mL, Lane 2: 1 μg/mL and Lane 3: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
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<strong>Figure 8 Immunohistochemistry Validation of CD4 in Human Thymus Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human thymus tissue using anti-CD4 antibody (PM-5201) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-mouse IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 1 Western Blot Validation with Human Recombinant Protein</strong><br> Loading: 5ng (Lane 1), 25ng (Lane 2) and 100ng (Lane 3) of human CD4 recombinant protein. Antibodies: CD4 PM-5201 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Observed at around 27kD.
<strong>Figure 2 Western Blot Validation in Human Thymus Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CD4 PM-5201 (Lane 1: 0.5 μg/mL, Lane 2: 1 μg/mL and Lane 3: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
<strong>Figure 3 Western Blot Validation in Human Jurkat Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CD4 PM-5201 (Lane 1: 1 μg/mL, Lane 2: 2 μg/mL and Lane 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
<strong>Figure 4 Western Blot Validation in Human, Mouse and Rat Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CD4 PM-5201 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
<strong>Figure 5 Western Blot Validation in Human Cell Lines </strong><br> Loading: 15 μg of lysates per lane. Antibodies: CD4 PM-5201 (4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
<strong>Figure 6 Western Blot Validation in Mouse Spleen Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CD4 PM-5201 (Lane 1: 1 μg/mL, Lane 2: 2 μg/mL and Lane 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
<strong>Figure 7 Western Blot Validation in Rat Lung Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: CD4 PM-5201 (Lane 1: 0.5 μg/mL, Lane 2: 1 μg/mL and Lane 3: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.
<strong>Figure 8 Immunohistochemistry Validation of CD4 in Human Thymus Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human thymus tissue using anti-CD4 antibody (PM-5201) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-mouse IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Documents

Further Information

Additional Names:
CD4 Antibody [9H5A8] : CD4mut
Application Note:
WB: 0.5-4 μg/mL; IHC: 5 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
CD4 Monoclonal Antibody: CD4 is a member of the immunoglobulin superfamily and is implicated as associative recognition element in MHC (Major Histocompatibility Complex) class II-restricted immune response. On T-lymphocytes, it defines the helper/inducer subset. The mature 55 kd CD4 protein consists of a 372 amino acid extracellular segment composed of four tandem immunoglobulin-like VJ regions. The CD4 molecule is a major receptor for human immunodeficiency virus (HIV), binding directly to the envelope glycoprotein gp120 on HIV, with the co-receptors being CCR5 or CXCR4. It has been shown that the V-like domains are critical for binding with HIV envelope gp120.
Background References:
  • Bowers et al. Int. J. Biochem. Cell Biol. 1997; 29 :871-5.
  • Arthos et al. Cell 1989; 57:469-81.
Buffer:
CD4 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Mouse monoclonal CD4 antibody was raised against a 193 amino acid recombinant protein from near the amino terminus of human CD4.
ISOFORMS:
Human CD4 has one isoform (458aa, 51kD). Mouse CD4 has two isoforms, including isoform 1 (457aa, 51kD) and isoform 2 (217aa, 24.5kD). Rat CD4 has one isoform (457aa, 51kD). PM-5201 can detect human, mouse and rat.
NCBI Gene ID #:
920
NCBI Official Name:
CD4 molecule
NCBI Official Symbol:
CD4
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 51kD

Observed: 51-57 kD (Post-modyfication: 2-4 N-linked glycosylations)
Protein Accession #:
NP_000607
Protein GI Number:
10835167
Purification:
CD4 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
Research Area:
Immunology
Swissprot #:
P01730
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Recombinant Protein Test (Figure 1): Anti-CD4 antibodies (PM-5201) detected human CD-4 recombinant protein at 5ng.

References

  1. Hood et al. Cytolytic nanoparticles attenuate HIV-1 infectivity. Antivir Ther. 2013;18(1):95-103. PMID: 22954649