ORAI1 Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-PM-5205-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-PM-5205-0.1mg | 0.1mg | £515.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 3F6H5
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
![Immunofluorescence of ORAI1 in human spleen tissue with ORAI1 antibody at 20 μg/ml.<br><br>Red: ORAI1 Antibody [3F6H5] (PM-5205) <br> Blue: DAPI staining](https://www.prosci-inc.com/static-images/ORAI1-Antibody-3F6H5_IF-2_PM-5205.gif "Immunofluorescence of ORAI1 in human spleen tissue with ORAI1 antibody at 20 μg/ml.<br><br>Red: ORAI1 Antibody [3F6H5] (PM-5205) <br> Blue: DAPI staining")
Documents
Further Information
Additional Names:
ORAI1 Antibody [3F6H5] : IMD9, ORAT1, CRACM1, TMEM142A
Application Note:
ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
ORAI1 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one fo rm of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.
Background References:
- Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.
- Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.
- Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.
Buffer:
ORAI1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Mouse monoclonal ORAI1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ORAI1.
NCBI Gene ID #:
84876
NCBI Official Name:
ORAI calcium release-activated calcium modulator 1
NCBI Official Symbol:
ORAI1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 33 kDa
Observed: 56 kDa
Observed: 56 kDa
Protein Accession #:
Q96D31
Protein GI Number:
97180269
Purification:
ORAI1 Monoclonal Antibody is Protein A purified.
Research Area:
Immunology
SPECIFICITY:
ORAI1 antibody is predicted to have no cross reactivity to other members in the ORAI family.
Swissprot #:
Q96D31
User NOte:
Optimal dilutions for each application to be determined by the researcher.