Lin28 Monoclonal Antibody

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ProSci
Product Code: PSI-PM-6143
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-PM-6143-0.02mg0.02mg£150.00
Quantity:
PSI-PM-6143-0.1mg0.1mg£515.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: 1G9H9
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of Lin28 in Raji cell lysate with Lin28 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Western blot analysis of Lin28 in Raji cell lysate with Lin28 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.

Documents

Further Information

Additional Names:
Lin28 Antibody [1G9H9] : CSDD1, LIN28, LIN-28, ZCCHC1, lin-28A
Application Note:
Lin28 antibody can be used for detection of Lin28 by Western blot at 0.5 - 1 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
Lin28 Monoclonal Antibody: Lin28 is a transcription factor that was first identified through its key role in the pathway of developmental timing in C. elegans. The role of Lin28 in development suggested that it might be useful in the creation of stem cells that might be beneficial in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing Lin28 in addition to the transcription factors POU5F1, Sox2, and NANOG in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing Lin28, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
Background References:
  • Ambros V. A hierarchy of regulatory genes controls a larva-to-adult developmental switch in C. elegans. Cell 1989; 57:49-57.
  • Carpenter MK, Rosler E, and Rao MS. Characterization and differentiation of human embryonic stem cells. Cloning Stem Cells 2003; 5:79-88.
  • Tyu J, Vodyanik MA, Smuga-Otto K, et al. Induced pluripotent stem cell lines derived from human somatic cells. Science 2007; 318:1917-20
  • Okita K, Nakagawa M, Hyenjong H, et al. Generation of mouse induced pluripotent stem cells without viral vectors. Science 2008; 322:949-53.
Buffer:
Lin28 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (87%), Chicken: (79%)
Immunogen:
Mouse monoclonal Lin28 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human Lin28.

The immunogen is located within the last 50 amino acids of Lin28.
NCBI Gene ID #:
79727
NCBI Official Name:
lin-28 homolog A (C. elegans)
NCBI Official Symbol:
LIN28A
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 23 kDa
Protein Accession #:
NP_078950
Protein GI Number:
13375938
Purification:
Lin28 Monoclonal Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
At least two isoforms of Lin28 are known to exist; this antibody will detect both. Lin28 antibody will not cross-react with Lin28 Homolog B.
Swissprot #:
Q9H9Z2
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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