SIRP alpha Antibody

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ProSci
Product Code: PSI-1125
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-1125-0.02mg0.02mg£150.00
Quantity:
PSI-1125-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of SIRP alpha in THP-1 whole cell lysate with SIRP alpha antibody at 0.5 μg/mL.
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Immunocytochemistry of SIRP alpha in THP-1 cells with SIRP alpha antibody at 1 μg/mL.
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Western blot analysis of SIRP alpha in THP-1 whole cell lysate with SIRP alpha antibody at 0.5 μg/mL.
Immunocytochemistry of SIRP alpha in THP-1 cells with SIRP alpha antibody at 1 μg/mL.

Documents

Further Information

Additional Names:
SIRP alpha Antibody: BIT, MFR, P84, SIRP, MYD-1, SHPS1, CD172A, PTPNS1, BIT, MYD1, Tyrosine-protein phosphatase non-receptor type substrate 1, Brain Ig-like molecule with tyrosine-based activation motifs, SHP substrate 1
Application Note:
SIRP alpha antibody can be used for Western blot at 0.5 - 1 μg/mL. 75 - 110 kDa bands should be detected. Antibody can also be used for immunocytochemistry starting at 1 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
SIRP alpha Antibody: Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPalpha, SHPS-1, MyD-1, BIT and p84. SIRPalpha is a new gene family containing at least fifteen members. SIRPalpha is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2. SIRPalpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes.
Background References:
  • Kharitonenkov A, et al. Nature 1997;386:181-186.
  • Fujioka Y, et al. Mol Cell Biol 1996;16:6887-6899
  • Yamao T, et al. Biochem Biophys Res Commun 1997;231:61-67
  • Brooke GP, et al. Eur J Immunol 1998;28:1-11
Buffer:
SIRP alpha Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (88%)
Immunogen:
SIRP alpha antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human SIRP alpha 1.

The immunogen is located within the last 50 amino acids of SIRP alpha.
NCBI Gene ID #:
140885
NCBI Official Name:
signal-regulatory protein alpha
NCBI Official Symbol:
SIRPA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
75 - 110 kDa
Protein Accession #:
NP_542970
Protein GI Number:
18426911
Purification:
SIRP alpha Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
Recognizes SIRP alpha 1, 2 and 3.
Swissprot #:
P78324
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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