NIK Antibody
| Code | Size | Price |
|---|
| PSI-1129-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-1129-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
NIK Antibody: HS, NIK, HSNIK, FTDCR1B, Mitogen-activated protein kinase kinase kinase 14, NF-kappa-beta-inducing kinase, HsNIK
Application Note:
NIK antibody can be used for detection of NIK by Western blot at 1 - 2 μg/mL.
Antibody validated: Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
NIK Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, LPS and mitogens. A serine/threonine protein kinase which mediates NF-κB activation by IL-1, TNFα and CD95 was identified recently and designated NIK (for NF-κB inducing kinase). NIK is an activator of IκB kinase alpha and beta (IKKα and IKKβ). Therefore, NIK is a key molecule in the NF-κB signaling pathway leading to the induction of a variety of gene expression in response to proinflammatory cytokines and bacteria products.
Background References:
- Malinin NL, Boldin MP, Kovalenko AV, et al. MAP3K-related kinase involved in NF-κB induction by TNF, CD95 and IL-1. Nature 1997; 385:540-4.
- Regnier CH, Song HY, Gao X, et al. Identification and characterization of an IκB kinase. Cell 1997; 90:373-83.
- Woronicz JD, Gao X, Cao Z, et al. IκB kinase-β: NF-κB activation and complex formation with IκB kinase-α and NIK. Science 1997; 278:866-9.
- Ling L, Cao Z, and Goeddel D. NF-κB-inducing kinase activates IKK-α by phosphorylation of Ser-176. Proc. Natl. Acad. Sci. USA 1998; 95:3792-7.
Buffer:
NIK Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (94%)
Immunogen:
NIK antibody was raised against a 17 amino acid peptide near the carboxy terminus of human NIK.
The immunogen is located within the last 50 amino acids of NIK.
The immunogen is located within the last 50 amino acids of NIK.
NCBI Gene ID #:
9020
NCBI Official Name:
mitogen-activated protein kinase kinase kinase 14
NCBI Official Symbol:
MAP3K14
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 104 kDa
Protein Accession #:
Q99558
Protein GI Number:
92090612
Purification:
NIK Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q99558
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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