GFR alpha 2 Antibody

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ProSci
Product Code: PSI-1135
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-1135-0.02mg0.02mg£150.00
Quantity:
PSI-1135-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of GFR alpha 2 in HeLa total cell lysate with GFR alpha 2 antibody at 1 μg/mL.
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Immunocytochemistry of GFR alpha 2 in HeLa cells with GFR alpha 2 antibody at 5 μg/mL.
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Western blot analysis of GFR alpha 2 in HeLa total cell lysate with GFR alpha 2 antibody at 1 μg/mL.
Immunocytochemistry of GFR alpha 2 in HeLa cells with GFR alpha 2 antibody at 5 μg/mL.

Documents

Further Information

Additional Names:
GFR alpha 2 Antibody: NTNRA, RETL2, TRNR2, GDNFRB, NRTNR-ALPHA, GDNF family receptor alpha-2, GDNF receptor beta, GDNF receptor alpha-2
Application Note:
GFR alpha 2 antibody can be used for detection of GFR alpha 2 by Western blot at 1 μg/mL. A 52 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
GFR alpha 2 Antibody: Members of the glial cell line-derived neurotrophic factor (GDNF) family, including GDNF and neurturin (NTN) play key roles in the control of vertebrate neuron survival and differentiation. Physiological responses to NTN require the presence of a novel glycosylphosphadidylinositol linked protein NTNRalpha, which is a cell surface receptor for NTN. The cDNAs encoding NTNRalpha from human, rat, chicken, and mouse have been cloned recently. NTNRalpha was also termed GDNFRβ, Ret ligand 2 (RETL2) or TGFbeta-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFRα-2 recently. GFRα-2 binds NTN and mediates activation of RET receptor tyrosine kinase by both NTN and GDNF. Thus, NTN, GFRα-2, and the Ret PTK form a complex to transduce NTN signal and to mediate NTN function.
Background References:
  • Kotzbauer PT, Lampe PA, Heuckeroth RO, et al. Neurturin, a relative glial-cell-line-derived neurotrophic factor. Nature 1996; 384:467-70.
  • Klein RD, Sherman D, Ho WH, et al. A GPI-linked protein that interacts with Ret to form a candidate neurturin receptor. Nature 1997; 387:717-21.
  • Buj-Bello A, Adu J, Pinon LG, et al. Neurturin responsiveness requires a GPI-linked receptor and the Ret receptor tyrosine kinase. Nature 1997; 387:721-4.
  • Baloh RH, Tansey MG, Golden JP, et al. TrnR2, a novel receptor that mediates neurturin and GDNF signaling through Ret. Neuron 1997; 18:793-802.
Buffer:
GFR alpha 2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (93%)
Immunogen:
GFR alpha 2 antibody was raised against a 15 amino acid peptide near the center of human GFR alpha 2.

The immunogen is located within amino acids 360 - 410 of GFR alpha 2.
NCBI Gene ID #:
2675
NCBI Official Name:
GDNF family receptor alpha 2
NCBI Official Symbol:
GFRA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 51 kDa

Observed: 52 kDa
Protein Accession #:
AAC52036
Protein GI Number:
2145080
Purification:
GFR alpha 2 Antibody is Ion exchange chromatography purified.
Research Area:
Growth Factors,Neuroscience
Swissprot #:
O00451
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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