FLIP Antibody

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ProSci
Product Code: PSI-1156
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-1156-0.02mg0.02mg£150.00
Quantity:
PSI-1156-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of FLIP in K562 cell lysate with FLIP antibody at (A) 1 and (B) 2 μg/ml.
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Immunocytochemistry of FLIP in 3T3 cells with FLIP antibody at 5 μg/mL.
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Western blot analysis of FLIP in K562 cell lysate with FLIP antibody at (A) 1 and (B) 2 μg/ml.
Immunocytochemistry of FLIP in 3T3 cells with FLIP antibody at 5 μg/mL.

Documents

Further Information

Additional Names:
FLIP Antibody: CASH, FLIP, MRIT, CLARP, FLAME, Casper, FLAME1, c-FLIP, FLAME-1, I-FLICE, c-FLIPL, c-FLIPR, c-FLIPS, CASP8AP1, CASH, CASP8 and FADD-like apoptosis regulator, Caspase homolog
Application Note:
FLIP antibody can be used for the detection of FLIP by Western blot at 1-2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
Background:
FLIP Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED-3 protease family.Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and MRIT. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1.
Background References:
  • Thome M, Schneider P, Hofmann K, Fickenscher H, Meinl E, Neipel F, Mattmann C, Burns K, Bodmer JL, Schroter M, Scaffidi C, Krammer PH, Peter ME, Tschopp J. Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors. Nature 1997;386:517-521.
  • Irmler M, Thome M, Hahne M, Schneider P, Hofmann K, Steiner V, Bodmer JL, Schroter M, Burns K, Mattmann C, Rimoldi D, French LE, Tschopp J. Inhibition of death receptor signals by cellular FLIP. Nature 1997;388:190-195.
  • Shu HB, Halpin DR , Goeddel DV. Casper is a FADD- and caspase-related inducer of apoptosis. Immunity 1997;6:751-763.
  • Hu S, Vincenz C, Ni J, Gentz R, Dixit VM. I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J Biol Chem 1997;272:17255-17257.
Buffer:
FLIP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Human: (77%)
Immunogen:
FLIP antibody was raised against a 17 amino acid peptide near the carboxy terminus of mouse FLIP.

The immunogen is located within the last 50 amino acids of FLIP.
NCBI Gene ID #:
8837
NCBI Official Name:
CASP8 and FADD-like apoptosis regulator
NCBI Official Symbol:
CFLAR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 53 kDa

Observed: 58 kDa
Protein Accession #:
AAC51622
Protein GI Number:
2253679
Purification:
FLIP Antibody is Antibody is DEAE purified.
Research Area:
Apoptosis
SPECIFICITY:
FLIP has short form (FLIPS) and long form (FLIPL) and antibody recognizes the FLIPL only.
Swissprot #:
O15519
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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