FLIP Antibody

Contact us
ProSci
Product Code: PSI-1161
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-1161-0.02mg0.02mg£150.00
Quantity:
PSI-1161-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 4
Western blot analysis of FLIP in HeLa (H), Jurkat (J), and K562 (K) whole cell lysate with FLIP antibody at 1:1000 dilution.
2 / 4
Immunocytochemistry of FLIP in Jurkat cells with FLIP antibody at 10 μg/mL.
3 / 4
Immunofluorescence of FLIP in Jurkat cells with FLIP antibody at 10 μg/mL.
4 / 4
Immunofluorescence of FLIP in K562 cells with FLIP antibody at 5 μg/mL.<br><br>Red: FLIP Antibody (1161) <br> Blue: DAPI staining
❮❯

Western blot analysis of FLIP in HeLa (H), Jurkat (J), and K562 (K) whole cell lysate with FLIP antibody at 1:1000 dilution.
Immunocytochemistry of FLIP in Jurkat cells with FLIP antibody at 10 μg/mL.
Immunofluorescence of FLIP in Jurkat cells with FLIP antibody at 10 μg/mL.
Immunofluorescence of FLIP in K562 cells with FLIP antibody at 5 μg/mL.<br><br>Red: FLIP Antibody (1161) <br> Blue: DAPI staining

Documents

Further Information

Additional Names:
FLIP Antibody: CASH, FLIP, MRIT, CLARP, FLAME, Casper, FLAME1, c-FLIP, FLAME-1, I-FLICE, c-FLIPL, c-FLIPR, c-FLIPS, CASP8AP1, CASH, CASP8 and FADD-like apoptosis regulator, Caspase homolog
Application Note:
Flip antibody can be used for detection of FLIP by Western blot at 1 μg/mL. A 55 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
FLIP Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively.
Background References:
  • Thome M, Schneider P, Hofmann K, et al. Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors. Nature 1997;386:517-521
  • Irmler M, Thome M, Hahne M, et al. Inhibition of death receptor signals by cellular FLIP. Nature 1997;388:190-195
  • Shu HB, Halpin DR , Goeddel DV. Casper is a FADD- and caspase-related inducer of apoptosis. Immunity 1997;6:751-763
  • Hu S, Vincenz C, Ni J, Gentz R, Dixit VM. I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J Biol Chem 1997;272:17255-17257
Buffer:
FLIP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
FLIP antibody was raised against a peptide corresponding to amino acids near the C-terminus of human FLIPaFLIPl form.

The immunogen is located within the last 50 amino acids of FLIP.
NCBI Gene ID #:
8837
NCBI Official Name:
CASP8 and FADD-like apoptosis regulator
NCBI Official Symbol:
CFLAR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
55 kDa
Protein Accession #:
AAC51622
Protein GI Number:
2253679
Purification:
FLIP Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
SPECIFICITY:
Antibody recognizes the FLIPa only.
Swissprot #:
O15519
User NOte:
Optimal dilutions for each application to be determined by the researcher.

Related Products

Product NameProduct CodeSupplier 
FLIP Peptide PSI-1161PProSci Summary Details