DR4 Antibody
Code | Size | Price |
---|
PSI-1167-0.02mg | 0.02mg | £150.00 |
PSI-1167-0.1mg | 0.1mg | £449.00 |
Overview
- Human
- Mouse
- Rat
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Images
Documents
Further Information
Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested.
- Pan et al. Science 1997;276:111-3
- Wiley et al. Immunity 1995; 3:673-82
- Pitti et al. J. Biol. Chem. 1996;271:12687-90
- Schneider et al. Immunity 1997; 7:831-6
The immunogen is located within the first 50 amino acids of DR4.
Observed: 55kD (Post-modification: 1 N-linked glycosylation)
KO Validation (Figure 1): Anti-DR4 antibodies (1167) specificity was further verified by DR4 specific knockout cell lysate. DR4 signal was detected in wild type cells, but not in the DR4 knockout cells.
Independent Antibody Validation in Cell lines (Figure 2) shows similar DR4 expression profile in human cell lines detected by two independent anti-DR4 antibodies that recognize different epitopes, 1139 against C-terminus domain and 1167 against the N-terminus domain. DR4 proteins are detected in all the tested cell lines except CaCo-2 at different expression levels by the two independent antibodies.
KD validation (Figure 6, 10): Anti-DR4 antibody specificity was further verified by DR4 specific siRNA knockdown. DR4 signal in SW480 and HeLa cells transfected with DR4 siRNAs was disrupted in comparison with that in cells transfected with control siRNAs.
References
- Goda et al. Mechanisms of enhancement of TRAIL tumoricidal activity against human cancer cells of different origin by dipyridamole. Oncogene. 2008 ;27(24):3435-45. PMID: 18193086
- Cantarella et al. Ischemic tolerance modulates TRAIL expression and its receptors and generates a neuroprotected phenotype. Cell Death Dis. 2014 ;5:e1331. PMID: 25032854
- Ekmekcioglu et al. Killing of human melanoma cells induced by activation of class I interferon-regulated signaling pathwaysvia MDA-7/IL-24. Cytokine. 2008;43(1):34-44.PMID: 18511292
- Devetzi et al. Death receptor 5 (DR5) and a 5-gene apoptotic biomarker panel with significant differential diagnostic potential in colorectal cancer. Sci Rep. 2016;6:36532. PMID: 27827395
- Horinaka et al. Luteolin induces apoptosis via death receptor 5 upregulation in human malignant tumor cells. Oncogene. 2005;24(48):7180-9. PMID: 16007131
- Malhi et al. Free fatty acids sensitise hepatocytes to TRAIL mediated cytotoxicity. Gut. 2007;56(8):1124-31. PMID: 17470478
- Kalan et al. Activation of the p53 Transcriptional Program Sensitizes Cancer Cells to Cdk7 Inhibitors. Cell Rep. 2017;21(2):467-48 PMID: 29020632
- Kurita et al. GLI3-dependent repression of DR4 mediates hedgehog antagonism of TRAIL-induced apoptosis. Oncogene. 2010;29(34):4848-58.PMID: 20562908
- Potu et al. Usp5 links suppression of p53 and FAS levels in melanoma to the BRAF pathway. Oncotarget. 2014;5(14):5559-69. PMID: 24980819
- Ashley et al. In vitro sensitivity testing of minimally passaged and uncultured gliomas with TRAIL and/or chemotherapy drugs. Br J Cancer. 2008;99(2):294-304. PMID: 18594532
- Cazanave et al. Death receptor 5 signaling promotes hepatocyte lipoapoptosis. J Biol Chem. 2011;286(45): 39336-48. PMID: 21941003
- Song et al. ABT-737 induces expression of the death receptor 5 and sensitizes human cancer cells to TRAIL-induced apoptosis. J Biol Chem. 2008;283(36):25003-13.PMID: 18599488
- Yoo et al. Effect of hyperthermia on TRAIL-induced apoptotic death in human colon cancer cells: development of a novel strategy for regional therapy. J Cell Biochem. 2007;101(3):619-30. PMID: 17212362
- Lauricella et al. SAHA/TRAIL combination induces detachment and anoikis of MDA-MB231 and MCF-7 breast cancer cells. Biochimie. 2012;94(2):287-99. PMID: 21835222
- Cantarella et al. Protective effects of the sigma agonist Pre-084 in the rat retina. Br J Ophthalmol. 2007;91(10):1382-4. PMID: 17522150
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