FLIP Antibody

ProSci
Product Code: PSI-2055
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-2055-0.02mg0.02mg£150.00
Quantity:
PSI-2055-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 5
Western blot analysis of FLIP in total cell lysates from HeLa (H), Jurkat (J), THP-1 (T), A431 (A), K562 (K) and NIH3T3 (N) cells with FLIP antibody at 1:1000 dilution.
2 / 5
Immunocytochemistry staining of HeLa cells using FLIP antibody at 2 μg/mL.
3 / 5
Immunofluorescence of FLIP in HeLa cells with FLIP antibody at 10 μg/mL.
4 / 5
Immunofluorescence of FLIP in Jurkat cells with FLIP antibody at 20 μg/mL.<br><br>Red: FLIP Antibody (2055) <br> Blue: DAPI staining
5 / 5
Immunocytochemistry of FLIP in Jurkat cells with FLIP antibody at 2 μg/mL.

Western blot analysis of FLIP in total cell lysates from HeLa (H), Jurkat (J), THP-1 (T), A431 (A), K562 (K) and NIH3T3 (N) cells with FLIP antibody at 1:1000 dilution.
Immunocytochemistry staining of HeLa cells using FLIP antibody at 2 μg/mL.
Immunofluorescence of FLIP in HeLa cells with FLIP antibody at 10 μg/mL.
Immunofluorescence of FLIP in Jurkat cells with FLIP antibody at 20 μg/mL.<br><br>Red: FLIP Antibody (2055) <br> Blue: DAPI staining
Immunocytochemistry of FLIP in Jurkat cells with FLIP antibody at 2 μg/mL.

Further Information

Additional Names:
FLIP Antibody: CASH, FLIP, MRIT, CLARP, FLAME, Casper, FLAME1, c-FLIP, FLAME-1, I-FLICE, c-FLIPL, c-FLIPR, c-FLIPS, CASP8AP1, CASH, CASP8 and FADD-like apoptosis regulator, Caspase homolog
Application Note:
FLIP can be used for detection of FLIP by Western blot at 1:1000 dilution. 35 kDa and 25 kDa bands can be detected. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
FLIP Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and c-FLIPs, respectively. The human FLIPs were also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and usurpin. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively.
Background References:
  • Thome M, Schneider P, Hofmann K, Fickenscher H, Meinl E, Neipel F, Mattmann C, Burns K, Bodmer JL, Schroter M, Scaffidi C, Krammer PH, Peter ME, Tschopp J. Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors. Nature 1997;386:517-521
  • Irmler M, Thome M, Hahne M, Schneider P, Hofmann K, Steiner V, Bodmer JL, Schroter M, Burns K, Mattmann C, Rimoldi D, French LE, Tschopp J. Inhibition of death receptor signals by cellular FLIP. Nature 1997;388:190-195
  • Shu HB, Halpin DR , Goeddel DV. Casper is a FADD- and caspase-related inducer of apoptosis. Immunity 1997;6:751-763
  • Hu S, Vincenz C, Ni J, Gentz R, Dixit VM. I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J Biol Chem 1997;272:17255-17257
Buffer:
FLIP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
FLIP antibody was raised against a 19 amino acid peptide near the carboxy terminus of human FLIP.

The immunogen is located within amino acids 180 - 230 of FLIP.
NCBI Gene ID #:
8837
NCBI Official Name:
CASP8 and FADD-like apoptosis regulator
NCBI Official Symbol:
CFLAR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
35 kDa and 25 kDa
Protein Accession #:
AAC51622
Protein GI Number:
2253679
Purification:
FLIP Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
SPECIFICITY:
Antibody recognizes human and murine FLIPgamma and FLIPdelta but not FLIPalpha and FLIPbeta.
Swissprot #:
O15519
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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