DNase II Antibody
| Code | Size | Price |
|---|
| PSI-2059-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-2059-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
DNase II Antibody: DNL, DNL2, DNASE2A, Deoxyribonuclease-2-alpha, Acid DNase, DNase II alpha
Application Note:
DNase II antibody can be used for detection of DNase II expression by Western blot at 0.5 μg/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
DNase II Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus. DNase II causes both chromatin condensation and DNA fragmentation. The genes encoding human, porcine, and murine DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (alpha) and 12 kDa (beta) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation. DNase II is ubiquitously expressed in human tissues.
Background References:
- Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999;401:127-8
- Yasuda T, Takeshita H, Iida R, Nakajima T, Hosomi O, Nakashima Y, Kishi K. Molecular cloning of the cDNA encoding human deoxyribonuclease II. J Biol Chem 1998;273:2610-6
- Krieser RJ, Eastman A. The cloning and expression of human deoxyribonuclease II. A possible role in apoptosis. J Biol Chem 1998;273:30909-14
- Shiokawa D, Tanuma S. Cloning of cDNAs encoding porcine and human DNase II. Biochem Biophys Res Commun 1998;247:864-9
Buffer:
DNase II Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
DNase II antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DNase II.
The immunogen is located within the last 50 amino acids of DNase II.
The immunogen is located within the last 50 amino acids of DNase II.
NCBI Gene ID #:
1777
NCBI Official Name:
deoxyribonuclease II, lysosomal
NCBI Official Symbol:
DNASE2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Aprox: 40 kDa
Protein Accession #:
AF047016
Protein GI Number:
2921838
Purification:
DNase II Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Signal Transduction
Swissprot #:
O00115
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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