ZIPK Antibody
| Code | Size | Price |
|---|
| PSI-2067-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-2067-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ZIPK Antibody: ZIP, ZIPK, Death-associated protein kinase 3, DAP-like kinase, DAP kinase 3
Application Note:
ZIPK antibody can be used for detection of DNase II expression by Western blot at 1 μg/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
ZIPK Antibody: Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase. ZIP kinase contains an N-terminal kinase domain and a C-terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREB family. ZIP kinase has high sequence homology to DAP kinase (death-associated protein kinase), which is a mediator of apoptosis induced by gamma interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues.
Background References:
- Kawai T, Matsumoto M, Takeda K, Sanjo H, Akira S. ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. Mol Cell Biol 1998;18:1642-51 (RD1299)
Buffer:
ZIPK Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ZIP kinase antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase.
The immunogen is located within amino acids 270 - 320 of ZIPK.
The immunogen is located within amino acids 270 - 320 of ZIPK.
NCBI Gene ID #:
1613
NCBI Official Name:
death-associated protein kinase 3
NCBI Official Symbol:
DAPK3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 26 kDa
Observed: 27 kDa
Observed: 27 kDa
Protein Accession #:
BAA81746
Protein GI Number:
5162884
Purification:
ZIPK Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Autophagy,Cancer
Swissprot #:
O43293
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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