CIDE-A Antibody

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ProSci
Product Code: PSI-2085
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-2085-0.02mg0.02mg£150.00
Quantity:
PSI-2085-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of CIDE-A in human brain tissue lysate in the absence (A) or presence (B) of peptide (2085P) with CIDE-A antibody at 1:2000 dilution.
2 / 3
Immunohistochemistry of CIDE-A in human brain tissue with CIDE-A antibody at 5 μg/mL.
3 / 3
Immunofluorescence of CIDE-A in Human Brain cells with CIDE-A antibody at 20 μg/mL.
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Western blot analysis of CIDE-A in human brain tissue lysate in the absence (A) or presence (B) of peptide (2085P) with CIDE-A antibody at 1:2000 dilution.
Immunohistochemistry of CIDE-A in human brain tissue with CIDE-A antibody at 5 μg/mL.
Immunofluorescence of CIDE-A in Human Brain cells with CIDE-A antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
CIDE-A Antibody: CIDE-A, Cell death activator CIDE-A, Cell death-inducing DFFA-like effector A
Application Note:
CIDE-A antibody can be used for detection of CIDE-A by Western blot at 2 μg/mL. An approximately 23 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
CIDE-A Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors. Cell death signals are transduced by DD-, DED-, or CARD-containing molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase DFF40/CAD, which is chaperoned and inhibited by DFF45/ICAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE-A is expressed in many tissues.
Background References:
  • Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184 2. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50
  • Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99
  • Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6
  • Inohara N, Koseki T, Chen S, Wu X, Nunez G. CIDE, a novel family of cell death activators with homology to the 45 kDa subunit of the DNA fragmentation factor. EMBO J 1998;17:2526-33
Buffer:
CIDE-A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CIDE-A antibody was raised against a 18 amino acid peptide near the carboxy terminus of human CIDE-A.

The immunogen is located within the last 50 amino acids of CIDE-A.
NCBI Gene ID #:
1149
NCBI Official Name:
cell death-inducing DFFA-like effector a
NCBI Official Symbol:
CIDEA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
23 kDa
Protein Accession #:
AF041378
Protein GI Number:
3114595
Purification:
CIDE-A Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
SPECIFICITY:
It has no cross activity to CIDE-B.
Swissprot #:
O60543
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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