IKK alpha Antibody
Code | Size | Price |
---|
PSI-2117-0.02mg | 0.02mg | £150.00 |
Quantity:
PSI-2117-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Images
Documents
Further Information
Additional Names:
IKK alpha Antibody: IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase alpha
Application Note:
IKK alpha antibody can be used for detection of IKK alpha by Western blot 0.5 μg/mL. An 85 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
IKK alpha Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα and bacteria product LPS. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The long-sought IκB kinase (IKK), which phosphorylates IκB, and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα and IKKβ). IKKα and IKKβ interact with each other and both are essential for the NF-κB activation. IKKα specifically phosphorylates IκB-alpha. IKKα is expressed in a variety of human tissues.
Background References:
- DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive IκB kinase that activates the transcription factor NF-κB. Nature 1997;388:548-54
- Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M. Identification and characterization of an IκB kinase. Cell 1997;90:373-83
- Zandi E, Rothwarf DM, Delhase M, Hayakawa M, Karin M. The IκB kinase complex (IKK) contains two kinase subunits, IKKα and IKKα, necessary for IκB phosphorylation and NF-κB activation. Cell 1997;91:243-52
- Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DY. IκB kinase-β: NF-κB activation and complex formation with IκB kinase-α and NIK. Science 1997;278:866-9
Buffer:
IKK alpha Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Mouse: (100%)
Immunogen:
IKK alpha antibody was raised against a 17 amino acid peptide near the carboxy terminus of human IKK alpha.
The immunogen is located within amino acids 640 - 690 of IKK alpha.
The immunogen is located within amino acids 640 - 690 of IKK alpha.
NCBI Gene ID #:
1147
NCBI Official Name:
conserved helix-loop-helix ubiquitous kinase
NCBI Official Symbol:
CHUK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 87 kDa
Protein Accession #:
AF009225
Protein GI Number:
2327068
Purification:
IKK alpha Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
This polyclonal antibody has no cross response to IKKb or IKKy.
Swissprot #:
O15111
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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