Bcl-10 Antibody

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ProSci
Product Code: PSI-2161
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-2161-0.02mg0.02mg£150.00
Quantity:
PSI-2161-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of Bcl-10 in Raji whole cell lysate in the absence (A) or presence (B) of peptide (2161P) with Bcl-10 antibody at 1:500 dilution.
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Immunocytochemistry of Bcl10 in Raji cells with Bcl10 antibody at 1 μg/mL.
3 / 3
Immunofluorescence of Bcl-10 in Raji cells with Bcl-10 antibody at 10 μg/mL.
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Western blot analysis of Bcl-10 in Raji whole cell lysate in the absence (A) or presence (B) of peptide (2161P) with Bcl-10 antibody at 1:500 dilution.
Immunocytochemistry of Bcl10 in Raji cells with Bcl10 antibody at 1 μg/mL.
Immunofluorescence of Bcl-10 in Raji cells with Bcl-10 antibody at 10 μg/mL.

Documents

Further Information

Additional Names:
Bcl-10 Antibody: CLAP, mE10, CIPER, c-E10, CARMEN, CLAP, CARD-containing molecule enhancing NF-kappa-B, Bcl-10
Application Note:
Bcl-10 antibody can be used for detection of BCL10 by Western blot at 0.5 μg/mL. dilution. An approximately 31 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Bcl-10 Antibody: Apoptosis is related to many diseases including cancer. Cell death signals are transduced by death domain (DD) and caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include ARC, RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD containing protein was recently identified by several groups and designated Bcl10, CIPER, mE10, CARMEN, CLAP. Bcl10 is a cellular homolog of the equine herpesvirus-2 E-10 gene. Overexpression of Bcl10 induces JNK, p38, and NF-κB activation. Bcl10 interacts with caspase-9 and enhances pro-caspase-9 processing and induces apoptosis through caspase-9 activation. Bcl10 exhibits a variety of mutations in MALT lymphomas and in B and T cell lineage lymphomas indicating that it may be commonly involved in the pathogenesis of human malignancy. Bcl10 is expressed in many human and murine tissues and cell lines.
Background References:
  • Willis TG, Jadayel DM, Du MQ, et al. Bcl10 is involved in t(1;14)(p22;q32) of MALT B cell lymphoma and mutated in multiple tumor types. Cell 1999;96(1):35-45
  • Koseki T, Inohara N, Chen S, et al. CIPER, a novel NF κB-activating protein containing a caspase recruitment domain with homology to Herpesvirus-2 protein E10. J Biol Chem 1999;274(15):9955-61
  • Yan M, Lee J, Schilbach S, Goddard A, Dixit V. mE10, a novel caspase recruitment domain-containing proapoptotic molecule. J Biol Chem 1999;274(15):10287-92
  • Thome M, Martinon F, Hofmann K, et al. Equine herpesvirus-2 E10 gene product, but not its cellular homologue, activates NF-κB transcription factor and c-Jun N-terminal kinase. J Biol Chem 1999;274(15):9962-8
Buffer:
Bcl-10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Bcl10 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Bcl10.

The immunogen is located within the first 50 amino acids of Bcl-10.
NCBI Gene ID #:
8915
NCBI Official Name:
B-cell CLL/lymphoma 10
NCBI Official Symbol:
BCL10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
31 kDa
Protein Accession #:
AF134395
Protein GI Number:
5070371
Purification:
Bcl-10 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Cancer
Swissprot #:
O95999
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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