Acinus Antibody

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ProSci
Product Code: PSI-2219
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-2219-0.02mg0.02mg£150.00
Quantity:
PSI-2219-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Immunofluorescence of Acinus in K562 cells with Acinus antibody at 20 μg/ml.<br><br>Green: Acinus Antibody (2219) <br> Blue: DAPI staining
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Immunofluorescence of Acinus in K562 cells with Acinus antibody at 20 μg/mL.
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Immunocytochemistry of Acinus in K562 cells with Acinus antibody at 5 μg/mL.
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Western blot analysis of Acinus in K562 whole cell lysate with Acinus antibody (IN) at 1 μg/mL.
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<strong>Figure 1. Immunofluorescence Validation of Acinus In HeLa Cells </strong><br> Immunofluorescent analysis of methanol-fixed HeLa cells labeling Acinus with 2219 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
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Immunofluorescence of Acinus in K562 cells with Acinus antibody at 20 μg/ml.<br><br>Green: Acinus Antibody (2219) <br> Blue: DAPI staining
Immunofluorescence of Acinus in K562 cells with Acinus antibody at 20 μg/mL.
Immunocytochemistry of Acinus in K562 cells with Acinus antibody at 5 μg/mL.
Western blot analysis of Acinus in K562 whole cell lysate with Acinus antibody (IN) at 1 μg/mL.
<strong>Figure 1. Immunofluorescence Validation of Acinus In HeLa Cells </strong><br> Immunofluorescent analysis of methanol-fixed HeLa cells labeling Acinus with 2219 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.

Documents

Further Information

Additional Names:
Acinus Antibody: ACN, ACINUS, fSAP152, KIAA0670, Apoptotic chromatin condensation inducer in the nucleus, Acinus
Application Note:
Acinus antibody can be used for detection of Acinus by Western blot at 1 μg/mL. A 220 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Acinus Antibody: Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE. A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS'.
Background References:
  • Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999 ;401:127-8.
  • Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto Y. Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Nature 1999 401:168-73.
Buffer:
Acinus Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (93%)
Immunogen:
Acinus antibody was raised against a 35 amino acid peptide near the center of human Acinus.

The immunogen is located within amino acids 760 - 810 of Acinus.
NCBI Gene ID #:
22985
NCBI Official Name:
apoptotic chromatin condensation inducer 1
NCBI Official Symbol:
ACIN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
220 kDa
Protein Accession #:
AAD56724
Protein GI Number:
5931959
Purification:
Acinus Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Cancer
Swissprot #:
Q9UKV3
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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