Survivin Antibody

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ProSci
Product Code: PSI-2233
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-2233-0.02mg0.02mg£150.00
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PSI-2233-0.1mg0.1mg£449.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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<strong>Figure 1 Western Blot Validation in Human MOLT4 Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Survivin 2233 (A: 1 μg/mL and B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Survivin 2233 (5 μg/mL), Survivin 2235 (4 μg/mL) and beta-actin 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Immunocytochemistry Validation of Survivin in Jurkat Cells</strong><br> Immunocytochemical analysis of Jurkat cells using anti-Survivin antibody (2233) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 3 Immunocytochemistry Validation of Survivin in Jurkat Cells</strong><br> Immunocytochemical analysis of Jurkat cells using anti-Survivin antibody (2233) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 5 Immunohistochemistry Validation of Survivin in Mouse Brain Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Survivin antibody (2233) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 1 Western Blot Validation in Human MOLT4 Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Survivin 2233 (A: 1 μg/mL and B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: Survivin 2233 (5 μg/mL), Survivin 2235 (4 μg/mL) and beta-actin 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Immunocytochemistry Validation of Survivin in Jurkat Cells</strong><br> Immunocytochemical analysis of Jurkat cells using anti-Survivin antibody (2233) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 3 Immunocytochemistry Validation of Survivin in Jurkat Cells</strong><br> Immunocytochemical analysis of Jurkat cells using anti-Survivin antibody (2233) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 5 Immunohistochemistry Validation of Survivin in Mouse Brain Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Survivin antibody (2233) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Documents

Further Information

Additional Names:
Survivin Antibody: API4, EPR-1, API4, IAP4, Baculoviral IAP repeat-containing protein 5, Apoptosis inhibitor 4
Application Note:
WB: 1-5μg/mL; ICC: 5 μg/mL; IF: 20 μg/mL; IHC: 5 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples; Immunofluorescence and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Background:
Survivin Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. A novel IAP protein was recently identified and designated survivin, apoptosis inhibitor 4 (API4), and TIAP. Survivin/TIAP interacted with the processed form of caspase-3 and inhibited its proteolytic activity. Survivin/TIAP is predominantly expressed in tissues of embryos, transformed cell lines, and many human cancers and lymphomas.
Background References:
  • Ambrosini et al. Nat Med 1997;3:917-21.
  • Ambrosini et al. Nat Med 1997;3:917-21.
  • Kobayashi et al. Proc Natl Acad Sci USA 1999;96:1457-62 (WD0600).
Buffer:
Survivin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (82%), Bovine: (82%), Rat: (58%)
Immunogen:
Anti-Survivin antibody (2233) was raised against a peptide corresponding to 12 amino acids near the amino terminus of human Survivin.

The immunogen is located within the first 50 amino acids of Survivin.
ISOFORMS:
Human Survivin has 7 isoforms, including isoform 1 (142aa, 16kD), isoform 2 (165aa, 19kD), isoform 3 (137aa, 16kD), isoform 4 (120aa, 14kD), isoform 5 (117aa, 14kD), isoform 6 (78aa, 9kD) and isoform 7 (74aa, 8.5kD). Mouse Survivin has 3 isoforms, including isoform 1 (140aa, 16kD), isoform 2 (121aa, 14kD) and isoform 3 (40aa, 4.7kD). Rat Survivin has one isoform (142aa, 17kD). 2233 can detect human and mouse.
NCBI Gene ID #:
332
NCBI Official Name:
baculoviral IAP repeat-containing 5
NCBI Official Symbol:
BIRC5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 17kD

Observed: 17 kD
Protein Accession #:
NP_001159
Protein GI Number:
59859878
Purification:
Survivin Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Cancer
Swissprot #:
O15392
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar Survivin expression profile in human cell lines detected by two independent anti-Survivin antibodies that recognize different epitopes, 2233 against N-terminus domain and 2235 against C-terminus domain.  Survivin proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

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