Caspase-12 Antibody
Code | Size | Price |
---|
PSI-2327-0.02mg | 0.02mg | £150.00 |
Quantity:
PSI-2327-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Images
Documents
Further Information
Additional Names:
Caspase-12 Antibody: Caspase-12, CASP-12
Application Note:
Caspase-12 antibody can be used for detection of caspase-12 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
Caspase-12 Antibody: Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta.
Background References:
- Nakagawa T, Zhu Human, Mouseorishima N, el al. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-β. Nature 2000; 403:98-103.
- Mehmet H. Caspases find a new place to hide. Nature 2000; 403:29-30
- Van de Craen M, Vandenabeele P, Declercq W,et al. Characterization of seven murine caspase family members. FEBS Lett 1997; 403:61-9.
Buffer:
Caspase-12 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Caspase-12 antibody was raised against a 17 amino acid peptide near the center of murine Caspase-12.
The immunogen is located within amino acids 90 - 140 of Caspase-12.
The immunogen is located within amino acids 90 - 140 of Caspase-12.
NCBI Gene ID #:
12364
NCBI Official Name:
caspase 12
NCBI Official Symbol:
Casp12
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 46 kDa
Observed: 55 kDa
Observed: 55 kDa
Protein Accession #:
CAA73532
Protein GI Number:
2094806
Purification:
Caspase-12 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Cancer
Swissprot #:
O08736
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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