p53R2 Antibody

Contact us
ProSci
Product Code: PSI-2383
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-2383-0.02mg0.02mg£150.00
Quantity:
PSI-2383-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 3
Western blot analysis of p53R2 in 3T3 cell lysate with p53R2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
2 / 3
Immunohistochemistry of p53R2 in human lung tissue with p53R2 antibody at 1 μg/mL.
3 / 3
Immunofluorescence of p53R2 in Human Lung tissue with p53R2 antibody at 20 μg/mL.
❮❯

Western blot analysis of p53R2 in 3T3 cell lysate with p53R2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of p53R2 in human lung tissue with p53R2 antibody at 1 μg/mL.
Immunofluorescence of p53R2 in Human Lung tissue with p53R2 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
p53R2 Antibody: P53R2, MTDPS8A, MTDPS8B, P53R2, Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53R2
Application Note:
p53R2 antibody can be used for detection of p53R2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
p53R2 Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including p53R2, Chk2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. p53R2 is a p53 inducible gene that contains a p53 binding sequence and encodes a subunit of the enzyme ribonucleotide reductase. p53R2 is induced by the reagents, ultraviolet and gamma-irradiation that cause DNA damages. The product of p53R2 gene is directly involved in the p53 checkpoint for repair of damaged DNA. The isoform of the p53 family member p73 also induces p53R2 expression. p53R2 is an important target of p53 for tumor suppression.
Background References:
  • Tanaka H, Arakawa H, Yamaguchi T, et al. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage. Nature 2000; 404:42-9.
  • Matsuoka S, Huang M, and Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science 1998;2 82:1893-7.
  • Oda E, Ohki R, Murasawa H, et al. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science 2000; 288:1053-8.
  • Oda K, Arakawa H, Tanaka T, et al. p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell 2000; 102:849-62.
Buffer:
p53R2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
p53R2 antibody was raised against a 16 amino acid peptide near the amino terminus of human p53R2.

The immunogen is located within the first 50 amino acids of p53R2.
NCBI Gene ID #:
50484
NCBI Official Name:
ribonucleotide reductase M2 B (TP53 inducible)
NCBI Official Symbol:
RRM2B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 39 kDa

Observed: 43 kDa
Protein Accession #:
BAA92434
Protein GI Number:
7229086
Purification:
p53R2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Cancer
SPECIFICITY:
At least six isoforms of p53R2 are known to exist; this antibody will detect all but the largest isoform.
Swissprot #:
Q7LG56
User NOte:
Optimal dilutions for each application to be determined by the researcher.

Related Products

Product NameProduct CodeSupplier 
p53R2 Peptide PSI-2383PProSci Summary Details