Chk2 Antibody
| Code | Size | Price |
|---|
| PSI-2391-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-2391-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
Chk2 Antibody: CDS1, CHK2, LFS2, RAD53, hCds1, HuCds1, PP1425, CDS1, CHK2 checkpoint homolog, Hucds1
Application Note:
Chk2 antibody can be used for detection of Chk2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Chk2 Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer.
Background References:
- Matsuoka S, Huang M, and Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science 1998; 282:1893-7.
- Tanaka H, Arakawa H, Yamaguchi T, et al. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage. Nature 2000; 404:42-9.
- Oda E, Ohki R, Murasawa H, et al. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science 2000; 288:1053-8.
- Oda K, Arakawa H, Tanaka T, et al. p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell 2000;102:849-62.
Buffer:
Chk2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Chk2 antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human Chk2.
The immunogen is located within the first 50 amino acids of Chk2.
The immunogen is located within the first 50 amino acids of Chk2.
NCBI Gene ID #:
11200
NCBI Official Name:
CHK2 checkpoint homolog (S. pombe)
NCBI Official Symbol:
CHEK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 60 kDa
Observed: 60 kDa
Observed: 60 kDa
Protein Accession #:
NP_009125
Protein GI Number:
6005850
Purification:
Chk2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
Swissprot #:
O96017
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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