Noxa Antibody
| Code | Size | Price |
|---|
| PSI-2437-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-2437-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
Noxa Antibody: Noxa, Phorbol-12-myristate-13-acetate-induced protein 1
Application Note:
Noxa antibody can be used for detection of Noxa by Western blot at at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Noxa Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes including p53R2, p53AIP1, and PUMA. A new p53 target gene, Noxa, was recently identified, which encodes a protein belonging to the subfamily of BH3-only proapoptic proteins. Noxa and PUMA are both transcriptional targets of p53 and BH3-only proteins. X-ray irradiation increased p53-dependent Noxa mRNA and protein levels. Noxa, when ectopically expressed, interacted with anti-apoptotic Bcl-2 family members, resulting in the activation of caspase-9. Noxa, like PUMA, localized to mitochondria and induces apoptosis in response to p53. Noxa and PUMA may represent direct mediators of p53-induced apoptosis. Increased levels of p53 and its target gene Noxa was found in the impaired tumor development.
Background References:
- Oda E, Ohki R, Murasawa H, et al. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science 2000; 288:1053-8.
- Nakano K and Vousden KH. PUMA, a novel proapoptotic gene, is induced by p53. Mol. Cell 2001; 7:683-94.
- Yu J, Zhang L, Hwang PM, et al. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol. Cell 2001; 7:673-82.
- Eferl R, Ricci R, Kenner L, et al. Liver tumor development. c-Jun antagonizes the proapoptotic activity of p53. Cell 2003; 112:181-92.
Buffer:
Noxa Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Noxa antibody was raised against a synthetic peptide corresponding to 17 amino acids at the amino terminus of mouse Noxa.
The immunogen is located within the first 50 amino acids of Noxa.
The immunogen is located within the first 50 amino acids of Noxa.
NCBI Gene ID #:
58801
NCBI Official Name:
phorbol-12-myristate-13-acetate-induced protein 1
NCBI Official Symbol:
Pmaip1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_067426
Protein GI Number:
10946832
Purification:
Noxa Antibody is immunoaffinity chromatography purified IgG.
Research Area:
Apoptosis,Cancer
Swissprot #:
Q9JM54
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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