PID Antibody

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ProSci
Product Code: PSI-2443
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-2443-0.02mg0.02mg£150.00
Quantity:
PSI-2443-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of PID expression in HeLa whole cell lysates in the absence (A) or presence (B) of blocking peptide with PID antibody at 1 μg/mL.
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Immunocytochemistry staining of HeLa using PID antibody at 10 μg/mL.
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Immunofluorescence of PID in HeLa cells with PID antibody at 10 ug/mL.
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Immunohistochemistry of PID in human small intestine tissue with PID antibody at 2.5 μg/ml.
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Western blot analysis of PID expression in HeLa whole cell lysates in the absence (A) or presence (B) of blocking peptide with PID antibody at 1 μg/mL.
Immunocytochemistry staining of HeLa using PID antibody at 10 μg/mL.
Immunofluorescence of PID in HeLa cells with PID antibody at 10 ug/mL.
Immunohistochemistry of PID in human small intestine tissue with PID antibody at 2.5 μg/ml.

Documents

Further Information

Additional Names:
PID Antibody: PID, MTA1L1, PID, Metastasis-associated protein MTA2, Metastasis-associated 1-like 1, MTA1-L1 protein
Application Note:
PID antibody can be used for detection of PID by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and Immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
PID Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 pathway, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The transcriptional activity of p53 is modulated by protein stability and acetylation. PID/MTA2, also termed MTA1-L1, was found to be a subunit of nucleosome remodeling and deacetylating (NRD/NuRD) complex. PID/MTA2 modulates the enzymatic activity of the histone deacetylase complex and its expression reduces the levels of acetylated p53. Deacetylation of p53 by PID/MTA2 represses p53-dependent transcriptional activation and modulates p53-mediated cell growth arrest and apoptosis. PID/MTA2 is ubiquitously expressed in human tissues.
Background References:
  • Matsuoka S, Huang M, and Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science 1998; 282:1893-7.
  • Tanaka H, Arakawa H, Yamaguchi T, et al. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage. Nature 2000; 404:42-9.
  • Oda E, Ohki R, Murasawa H, et al. Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis. Science 2000; 288:1053-8.
  • Oda K, Arakawa H, Tanaka T, et al. p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53. Cell 2000;102:849-62.
Buffer:
PID Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PID antibody was raised against a 17 amino acid peptide near the carboxy terminus of human PID.

The immunogen is located within the last 50 amino acids of PID.
NCBI Gene ID #:
9219
NCBI Official Name:
metastasis associated 1 family, member 2
NCBI Official Symbol:
MTA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 73 kDa

Observed: 75 kDa
Protein Accession #:
AAG02241
Protein GI Number:
9931638
Purification:
PID Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
SPECIFICITY:
PID antibody is predicted to not cross-react with MTA2
Swissprot #:
O94776
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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