CARD10 Antibody
| Code | Size | Price |
|---|
| PSI-3013-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-3013-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
CARD10 Antibody: BIMP1, CARMA3, Caspase recruitment domain-containing protein 10, CARD-containing MAGUK protein 3, Carma 3
Application Note:
CARD10 antibody can be used for detection of CARD10 by Western blot at 5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
CARD10 Antibody: Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, Bcl-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD10. This protein belongs to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARD 10 interacts with Bcl-10, a Bcl protein that promotes apoptosis, caspase-9 maturation and activation of NF-κB. CARD 10 and the related protein CARMA1 also associate with NEMO, the regulatory subunit of the IκK complex, demonstrating its importance in the regulation of NF-κB transcription factor activation.
Background References:
- Wang L, Guo Y, Huang W-J, et al. CARD10 is a novel caspase recruitment domain/membrane-associated guanylate kinase family member that interacts with BCL10 and activates NF-kappaB. J. Biol. Chem. 2001; 276:21405-9.
- Fanning AS and Anderson JM. Protein modules as organizers of membrane structure. Curr. Opin. Cell Biol. 1999; 11:432-9.
- Fischer KD, Tedford K, Wirth T. New roles for Bcl10 in B-cell development and LPS response. Trends Immunol. 2004; 25:113-6.
- Stilo R, Liguoro D, Di Jeso B, et al. Physical and functional interaction of CARMA1 and CARMA3 with Ikappa kinase kappa-NF-kappaB essential modulator. J. Biol. Chem. 2004; 279:34323-31.
Buffer:
CARD10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CARD10 antibody was raised against a synthetic peptide corresponding to 14 amino acids at the C-terminus of human CARD10.
The immunogen is located within the last 50 amino acids of CARD10.
The immunogen is located within the last 50 amino acids of CARD10.
NCBI Gene ID #:
29775
NCBI Official Name:
caspase recruitment domain family, member 10
NCBI Official Symbol:
CARD10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 116 kDa
Observed: 115 kDa
Observed: 115 kDa
Protein Accession #:
AAK26165
Protein GI Number:
13488607
Purification:
CARD10 Antibody is immunoaffinity purified .
Research Area:
Apoptosis
Swissprot #:
Q9BWT7
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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