NALP1 Antibody

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ProSci
Product Code: PSI-3037
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3037-0.02mg0.02mg£150.00
Quantity:
PSI-3037-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of NALP1 in U937 cell lysate with NALP1 antibody at (A) 1, (B) 2 and (C) 4 μg/mL.
2 / 3
Immunocytochemistry of NALP1 in K562 cells with NALP1 antibody at 10 μg/mL.
3 / 3
Immunofluorescence of NALP1 in K562 cells with NALP1 antibody at 20 μg/mL.
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Western blot analysis of NALP1 in U937 cell lysate with NALP1 antibody at (A) 1, (B) 2 and (C) 4 μg/mL.
Immunocytochemistry of NALP1 in K562 cells with NALP1 antibody at 10 μg/mL.
Immunofluorescence of NALP1 in K562 cells with NALP1 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
NALP1 Antibody: NAC, CARD7, CIDED, NALP1, SLEV1, DEFCAP, PP1044, VAMAS1, CLR17.1, DEFCAP-L/S, KIAA0926, NAC, NACHT, LRR and PYD domains-containing protein 1, Caspase recruitment domain-containing protein 7
Application Note:
NALP1 antibody can be used for detection of NALP1 by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
NALP1 Antibody: NALP1 belongs to a family of cytoplasmic proteins that have been implicated in cell responses to apoptotic and inflammatory stimuli. It contains a caspase recruitment domain (CARD) in addition to a NACHT domain and leucine rich repeat (LRR). This protein interacts strongly with caspase 2 and weakly with caspase 9, as well as with Apaf-1. Transient overexpression of this gene in cultured cells was sufficient to induce apoptosis. NALP1 mRNA and protein expression levels in myeloid leukemia cells are increased following CREB (cAMP-response element binding protein) activation, suggesting that NALP1 may contribute to modulate the response of these cells to pro-inflammatory stimuli. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for NALP1.
Background References:
  • NALP1 antibody can be stored at 4˚C, stable for one year. Tschopp J, Martinon F, and Burns K. NALPs: a novel protein family involved in inflammation. Nat. Rev. Mol. Cell Biol. 2003; 4:95-104.
  • Chu ZL, Pio F, Xie Z, et al. A novel enhancer of the Apaf1 apoptosome involved in cytochrome c-dependent caspase activation and apoptosis. J. Biol. Chem. 2001; 276:9239-45.
  • Hlaing T, Guo RF, Dilley KA, et al. Molecular cloning and characterization of DEFCAP-L and -S, two isoforms of a novel member of the Ced-4 family of apoptosis proteins. J. Biol. Chem. 2001; 276:9230-8.
  • Sanz C, Calasanz MJ, Andreu E, et al. NALP1 is a transcriptional target for cAMP-response-element-binding protein (CREB) in myeloid leukemia cells. Biochem. J. 2004; 384:281-6.
Buffer:
NALP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
NALP1 antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human NALP1.

The immunogen is located within the last 50 amino acids of NALP1.
NCBI Gene ID #:
22861
NCBI Official Name:
NLR family, pyrin domain containing 1
NCBI Official Symbol:
NLRP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAG30288
Protein GI Number:
11096303
Purification:
NALP1 Antibody is immunoaffinity chromatography purified IgG.
Research Area:
Apoptosis
Swissprot #:
Q9C000
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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