AATF Antibody

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ProSci
Product Code: PSI-3039
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3039-0.02mg0.02mg£150.00
Quantity:
PSI-3039-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of AATF in human small intestine cell lysate with AATF antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
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Immunohistochemistry of AATF in human small intestine tissue with AATF antibody at 10 μg/mL.
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Immunofluorescence of AATF in Human Small Intestine cells with AATF antibody at 20 μg/mL.
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Western blot analysis of AATF in human small intestine cell lysate with AATF antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Immunohistochemistry of AATF in human small intestine tissue with AATF antibody at 10 μg/mL.
Immunofluorescence of AATF in Human Small Intestine cells with AATF antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
AATF Antibody: DED, BFR2, CHE1, CHE-1, DED, HSPC277, Protein AATF, Apoptosis-antagonizing transcription factor
Application Note:
AATF antibody can be used for the detection of AATF by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
AATF Antibody: AATF (apoptosis antagonizing transcription factor) was initially discovered as an interaction partner of ZIP kinase (ZIPK), a member of death-associated protein (DAP) kinase family of pro-apoptotic serine/threonine kinases. AATF is a phosphoprotein containing an acidic region and a putative leucine zipper domain and nuclear localization signal, features which are typical of transcription factors. AATF inhibits the ZIPK-mediated pro-apoptotic pathway and may activate other anti-apoptotic pathways. Recently, it has also been shown to protect neural cells against oxidative damage induced by amyloid b-peptide and to inhibit aberrant production of the beta-peptide by interacting with Par-4 (prostate apoptosis response-4), another pro-apoptotic leucine zipper protein that is associated with neuronal degeneration in Alzheimer's disease (AD), suggesting that AATF may have potential therapeutic applications in both familial and sporadic forms of AD.
Background References:
  • AATF antibody can be stored at 4˚C, stable for one year. Page G, Lodige I, Kogel D et al. AATF, a novel transcription factor that interacts with Dlk/ZIP kinase and interferes with apoptosis. FEBS Lett. 1999; 462:187-191.
  • Kawai T, Matsumoto M, Takeda K, et al. ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. Mol. Cell Biol. 1998; 18:1642-51.
  • Lindfors K, Halttunen T, Huotari P, et al. Identification of novel transcription factor-like gene from human intestinal cells. Biochem. Biophys. Res. Commun. 2000; 276:660-6.
  • Xie J and Guo Q. AATF protects neural cells against oxidative damage induced by amyloid b-peptide. Neurobiol. of Dis. 2004; 16:150-7.
Buffer:
AATF Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
AATF antibody was raised against a 12 amino acid synthetic peptide from near the carboxy terminus of human AATF.

The immunogen is located within the last 50 amino acids of AATF.
NCBI Gene ID #:
26574
NCBI Official Name:
apoptosis antagonizing transcription factor
NCBI Official Symbol:
AATF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_036270
Protein GI Number:
7657013
Purification:
AATF Antibody is immunoaffinity purified IgG.
Research Area:
Apoptosis
Swissprot #:
Q9NY61
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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