OMI Antibody

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ProSci
Product Code: PSI-3051
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3051-0.02mg0.02mg£150.00
Quantity:
PSI-3051-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of OMI in U937 lysate with Omi antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
2 / 3
Immunohistochemistry of OMI in human liver tissue with OMI antibody at 2 μg/mL.
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Immunofluorescence of OMI in Human Liver cells with OMI antibody at 20 μg/mL.
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Western blot analysis of OMI in U937 lysate with Omi antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Immunohistochemistry of OMI in human liver tissue with OMI antibody at 2 μg/mL.
Immunofluorescence of OMI in Human Liver cells with OMI antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
OMI Antibody: OMI, PARK13, PRSS25, OMI, Serine protease HTRA2, mitochondrial, High temperature requirement protein A2, HtrA2
Application Note:
OMI antibody can be used for detection of OMI by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
OMI Antibody: Inhibitor of apoptosis proteins (IAPs) were initially identified in baculoviruses as proteins that inhibit apoptosis of the host cells to allow time for viral replication. Cellular homologues containing at least one baculoviral IAP repeat (BIR) motif essential for their anti-apoptosis activity have been identified in yeasts and higher organisms and often act by binding and inhibiting processed caspases. The activity of these proteins can be modulated by the expression of proteins such as Smac/DIABLO and XAF-1 which displace or prevent the binding of caspases by IAPs. Recently, a mitochondrial serine protease termed Omi/HtrA2 has been found to bind IAPs. Similar to Smac, Omi possesses a conserved IAP-binding motif, but acts to cleave IAPs to irreversibly inactivate IAPs and promote apoptosis.
Background References:
  • Crook NE, Clem RJ, and Miller LK. An apoptosis inhibiting baculovirus gene with a zinc finger like motif. J. Virol. 1993; 67:2168-2174.
  • Liston P, Fong WG, and Korneluk RG. The inhibitors of apoptosis: there is more to life than Bcl2. Oncogene 2003; 22:8568-80.
  • Vaux DL and Silke J. Mammalian mitochondrial IAP binding proteins. Biochem. Biophys. Res. Comm. 2003; 304:499-504.
  • Suzuki Y, Imai Y, Nakayama H, et al. A serine protease, HtrA2, is released from the mitochondria and interacts with XIAP, inducing cell death. Mol. Cell 2001; 8:613-21.
Buffer:
OMI Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
OMI antibody was raised against a peptide corresponding to 16 amino acids near the C-terminus of human Omi.

The immunogen is located within amino acids 70 - 120 of OMI.
NCBI Gene ID #:
27429
NCBI Official Name:
HtrA serine peptidase 2
NCBI Official Symbol:
HTRA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAB94569
Protein GI Number:
5870865
Purification:
OMI Antibody is Ion exchange chromatography purified.
Research Area:
Apoptosis
Swissprot #:
O43464
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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