AID Antibody
| Code | Size | Price |
|---|
| PSI-3091-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-3091-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
AID Antibody: AID, ARP2, CDA2, HIGM2, HEL-S-284, AID, Single-stranded DNA cytosine deaminase, Activation-induced cytidine deaminase
Application Note:
AID antibody can be used for detection of AID by Western blot at 2 μg/mL. Anti-AID is human and mouse reactive. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
AID Antibody: Activation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNA-editing cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines. It is necessary for somatic hypermutation and class switch recombination in B cells, but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms. Although it is structurally and functionally similar to the APOBEC proteins, it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G.
Background References:
- Muramatsu M, Sankaranand VS, Anant S, et al. Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells. J. Biol. Chem. 1999; 274:18470-6.
- Muramatsu M, Kinoshita K, Fagarasan S, et al. Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme.Cell 2000; 102:553-63.
- Greeve J, Philipsen A, Krause K, et al. Expression of activation-induced cytidine deaminase in human B-cell Hodgkin lymphomas. Blood 2003; 101:3574-80.
- Cascalho M. Advantages and disadvantages of cytidine deamination. J. Immunol. 2004; 172:6513-8.
Buffer:
AID Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
AID antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human AID.
The immunogen is located within the last 50 amino acids of AID.
The immunogen is located within the last 50 amino acids of AID.
NCBI Gene ID #:
57379
NCBI Official Name:
activation-induced cytidine deaminase
NCBI Official Symbol:
AICDA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 22 kDa
Observed: 25 kDa
Observed: 25 kDa
Protein Accession #:
AAM95402
Protein GI Number:
22297218
Purification:
AID Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
Swissprot #:
Q9GZX7
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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