ATR Antibody
| Code | Size | Price |
|---|
| PSI-3117-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-3117-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ATR Antibody: ATR, GAPO, TEM8, ATR, Anthrax toxin receptor 1, Tumor endothelial marker 8
Application Note:
ATR antibody can be used for detection of ATR by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
ATR Antibody: The Anthrax toxin receptor (ATR) was initially discovered as the tumor endothelial marker 8 (TEM8). This protein, which exists in three isoforms (36, 40, and 60 kDa), is highly expressed in tumor vessels as well as in the vasculature of developing embryos, suggesting that it may normally play a role in angiogenesis. However, it also acts as the receptor for anthrax toxin. Following the binding of this protein by the protective antigen (PA) of anthrax, PA is cleaved and heptamerizes to form the binding site for both edema factor (EF) and lethal factor (LF). This complex is then endocytosed by the cell; acidification in endosomes allows the release of EF and LF into the cytoplasm where they interfere with MAPK signaling and induce apoptosis.
Background References:
- Carson-Walter EB, Watkins DN, Nanda A, et al. Cell surface tumor endothelial markers are conserved in mice and humans. Can. Res. 2001; 61:6649-6655.
- Bradley KA, Mogridge J, Mourez M, et al. Identification of the cellular receptor for anthrax toxin. Nature 2001; 414:225-9.
- Molloy S, Bresnahan PA, Thomas G, et al. Human furin is a calcium-dependent serine endoprotease that recognizes the sequence Arg-X-X-Arg and efficiently cleaves anthrax toxin protective antigen. J. Biol. Chem. 1992; 267:16396-402.
- Duesbery N, Webb C, Vande Woude G, et al. Proteolytic inactivation of MAP-kinase-kinase by anthrax lethal factor. Science 1998; 280:734-6.
Buffer:
ATR Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ATR antibody was raised against a peptide corresponding to 13 amino acids near the center of human ATR.
The immunogen is located within amino acids 220 - 270 of ATR.
The immunogen is located within amino acids 220 - 270 of ATR.
NCBI Gene ID #:
84168
NCBI Official Name:
anthrax toxin receptor 1
NCBI Official Symbol:
ANTXR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 36, 40, 60 kDa
Observed: 45 kDa
Observed: 45 kDa
Protein Accession #:
NP_444262
Protein GI Number:
16933551
Purification:
ATR Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience,Infectious Disease
SPECIFICITY:
At least three isoforms of ATR are known to exist; this antibody will detect all three isoforms.
Swissprot #:
Q9H6X2
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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