PHAP III Antibody
| Code | Size | Price |
|---|
| PSI-3147-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-3147-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
PHAP III Antibody: LANPL, LANP-L, Acidic leucine-rich nuclear phosphoprotein 32 family member E, LANP-like protein
Application Note:
PHAP III antibody can be used for detection of PHAP III by Western blot at 1 μg/mL. A band at approximately 35 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human and rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human and rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
PHAP III Antibody: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.
Background References:
- Jiang X, Kim HE, Shu H, Zhao Y, Zhang H, Kofron J, Donnelly J, Burns D, Ng SC , Rosenberg S, Wang X. Distinctive roles of PHAP proteins and prothymosin-α in a death regulatory pathway. Science. 2003;299(5604):223-6.
- Nicholson DW, Thornberry NA. Apoptosis. Life and death decisions. Science. 2003 10;299(5604):214-5.
Buffer:
PHAP III Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PHAP III antibody was raised with a synthetic peptide corresponding to amino acids close to carboxy terminus of human PHAP III.
The immunogen is located within amino acids 200 - 250 of PHAP III.
The immunogen is located within amino acids 200 - 250 of PHAP III.
NCBI Gene ID #:
81611
NCBI Official Name:
acidic (leucine-rich) nuclear phosphoprotein 32 family, member E
NCBI Official Symbol:
ANP32E
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
35 kDa
Protein Accession #:
NP_112182
Protein GI Number:
13569879
Purification:
PHAP III Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
SPECIFICITY:
PHAP III has no cross-reaction to PHAP I and PHAP I2a.
Swissprot #:
Q9BTT0
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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