Caspase-12 Antibody (Small)

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ProSci
Product Code: PSI-3197
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3197-0.02mg0.02mg£150.00
Quantity:
PSI-3197-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of caspase-12 in mouse (lane A) and rat (lane B) liver lysate with caspase-12 antibody (small) at 1 μg/mL.
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Immunohistochemical staining of human liver tissue using caspase-12 antibody (small) at 10 μg/mL.
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Western blot analysis of caspase-12 in mouse (lane A) and rat (lane B) liver lysate with caspase-12 antibody (small) at 1 μg/mL.
Immunohistochemical staining of human liver tissue using caspase-12 antibody (small) at 10 μg/mL.

Documents

Further Information

Additional Names:
Caspase-12 Antibody (Small): Caspase-12, CASP-12
Application Note:
Caspase-12 (small) antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.

Antibody validated: Western Blot in mouse and rat samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
Caspase-12 Antibody: (Small)Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.
Background References:
  • Nakagawa T, Zhu Human, Mouseorishima N, el al. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-β. Nature 2000; 403:98-103.
  • Mehmet H. Caspases find a new place to hide. Nature 2000; 403:29-30
  • Van de Craen M, Vandenabeele P, Declercq W,et al. Characterization of seven murine caspase family members. FEBS Lett 1997; 403:61-9.
Buffer:
Caspase-12 Antibody is supplied in PBS containing 0.02% sodium azide. (Small)
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (93%)
Immunogen:
Caspase-12 antibody was raised against a synthetic peptide corresponding to 15 amino acids within the small cleavage product of murine caspase-12.

The immunogen is located within the last 50 amino acids of Caspase-12 .
NCBI Gene ID #:
12364
NCBI Official Name:
caspase 12
NCBI Official Symbol:
Casp12
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 46 kDa (uncleaved)

Observed: 38 kDa
Protein Accession #:
CAA73532
Protein GI Number:
2094806
Purification:
Caspase-12 Antibody (Small) is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Cancer
Swissprot #:
O08736
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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