TRIM5 gamma Antibody

Product Code: PSI-3239

Supplier: ProSci

Code	Size	Price

PSI-3239-0.02mg

0.02mg

£150.00

Quantity:

PSI-3239-0.1mg

0.1mg

£449.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Western Blot (WB)

Images

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Western blot analysis of TRIM5 gamma expression in human bladder (A) and colon (B) cell lysate with TRIM5 gamma antibody at 2 μg/ml.

2 / 3

Immunohistochemistry of TRIM5 gamma in human bladder tissue with TRIM5 gamma antibody at 2 μg/mL.

3 / 3

Immunofluorescence of TRIM5 gamma in human bladder tissue with TRIM5 gamma antibody at 20 μg/mL.

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Documents

Further Information

Additional Names:

TRIM5 gamma Antibody: RNF88, TRIM5alpha, RNF88, Tripartite motif-containing protein 5, RING finger protein 88

Application Note:

TRIM5 gamma antibody can be used for detection of TRIM5 gamma by Western blot at 2 μg/mL. A band at approximately 35 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Background:

TRIM5 gamma Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has five alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. While a function has not yet been assigned to TRIM5gamma, it is known that expression of TRIM5α variants from humans, rhesus monkeys, and African green monkeys enable resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. Furthermore, the TRIM5δ isoform appears to serve as a scaffold for the assembly of endogenous BTBD1 and BTBD2 proteins and also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner.

Background References:

Reymond A, Meroni G, Fantozzi A, et al. The tripartite motif family identifies cell compartments. EMBO J. 2001; 20:2140-51.
Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5a restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.
Hatziioannou T, Perez-Caballero D, Yang A, et al. Retrovirus resistance factors REF1 and Lv1 are species-specific variants of TRIM5α. Proc. Nat'l. Acad. Sci. USA 2004; 101:10774-9
Xu L, Yang L, Moitra PK, et al. BTBD1 and BTBD2 colocalize to cytoplasmic bodies with the RBCC/tripartite motif protein, TRIM5a. Exp. Cell Res. 2003; 288:84-93.

Buffer:

TRIM5 gamma Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration:

1 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Immunogen:

TRIM5 gamma antibody was raised against a synthetic peptide corresponding to 15 amino acids near the carboxy terminus of human TRIM5 gamma.

The immunogen is located within the last 50 amino acids of TRIM5 gamma.

NCBI Gene ID #:

85363

NCBI Official Name:

tripartite motif-containing 5

NCBI Official Symbol:

TRIM5

NCBI Organism:

Homo sapiens

Physical State:

Liquid

Protein Accession #:

NP_149083

Protein GI Number:

15011944

Purification:

TRIM5 gamma Antibody is affinity chromatography purified via peptide column.

Research Area:

Infectious Disease

Swissprot #:

Q9C035

User NOte:

Optimal dilutions for each application to be determined by the researcher.

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