TRIM5 alpha Antibody
| Code | Size | Price |
|---|
| PSI-3247-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-3247-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
TRIM5 alpha Antibody: RNF88, TRIM5alpha, RNF88, Tripartite motif-containing protein 5, RING finger protein 88
Application Note:
TRIM5 alpha antibody can be used for detection of TRIM5 alpha by Western blot at 2 μg/mL. A band at approximately 55 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
TRIM5 alpha Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5α variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5α variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5α acts as a natural barrier to cross-species retrovirus transmission.
Background References:
- Reymond A, Meroni G, Fantozzi A, et al. The tripartite motif family identifies cell compartments. EMBO J. 2001; 20:2140-51.
- Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5a restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.
- Hatziioannou T, Perez-Caballero D, Yang A, et al. Retrovirus resistance factors REF1 and Lv1 are species-specific variants of TRIM5α. Proc. Nat'l. Acad. Sci. USA 2004; 101:10774-9
Buffer:
TRIM5 alpha Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TRIM5 alpha antibody was raised against a synthetic peptide corresponding to amino acids near the mid-region of human TRIM5 alpha.
The immunogen is located within amino acids 370 - 420 of TRIM5 alpha.
The immunogen is located within amino acids 370 - 420 of TRIM5 alpha.
NCBI Gene ID #:
85363
NCBI Official Name:
tripartite motif-containing 5
NCBI Official Symbol:
TRIM5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_149023
Protein GI Number:
14719418
Purification:
TRIM5 alpha Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease
Swissprot #:
Q9C035
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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