APOBEC3G Antibody

Contact us
ProSci
Product Code: PSI-3257
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-3257-0.02mg0.02mg£150.00
Quantity:
PSI-3257-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 2
Western blot analysis of APOBEC3G expression in Caco-2 cell lysate in the (A), absence and (B) presence of blocking peptide with APOBEC3G antibody at 5 μg/mL.
2 / 2
Immunohistochemical staining of human spleen using APOBEC3G antibody at 1 μg/mL.
❮❯

Western blot analysis of APOBEC3G expression in Caco-2 cell lysate in the (A), absence and (B) presence of blocking peptide with APOBEC3G antibody at 5 μg/mL.
Immunohistochemical staining of human spleen using APOBEC3G antibody at 1 μg/mL.

Documents

Further Information

Additional Names:
APOBEC3G Antibody: A3G, ARCD, ARP9, ARP-9, CEM15, CEM-15, MDS019, bK150C2.7, dJ494G10.1APOBEC-related cytidine deaminase, APOBEC-related protein
Application Note:
APOBEC3G antibody can be used for detection of APOBEC3G by Western blot at 5 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
APOBEC3G Antibody: The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates. Similar to TRIM5α which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome. This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G; a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity.
Background References:
  • Jarmuz A, Chester A, Bayliss J, et al. An anthropoid-specific locus of Orphan C to U RNA-editing enzymes on chromosome 22. Genomics 2002; 79:285-96.
  • Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5a restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.
  • Bieniasz PD. Intrinsic immunity: a front-line defense against viral attack. Nat Immunol. 2004; 5:1109-15.
  • Sheehy AM, Gaddis NC, Choi JD, et al. Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein. Nature 2002; 418:646-50.
Buffer:
APOBEC3G Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
APOBEC3G antibody was raised against a synthetic peptide corresponding to 15 amino acids near the amino-terminus of human APOBEC3G.

The immunogen is located within the first 50 amino acids of APOBEC3G.
NCBI Gene ID #:
60489
NCBI Official Name:
apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G
NCBI Official Symbol:
APOBEC3G
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_068594
Protein GI Number:
13399304
Purification:
APOBEC3G Antibody is affinity chromatography purified via peptide column.
Research Area:
Infectious Disease
SPECIFICITY:
APOBEC3G antibody will also detect the APOBEC3F isoform.
Swissprot #:
Q9HC16
User NOte:
Optimal dilutions for each application to be determined by the researcher.

Related Products

Product NameProduct CodeSupplier 
APOBEC3G PeptidePSI-3257PProSci Summary Details