MD-2 Antibody
Code | Size | Price |
---|
PSI-3289-0.02mg | 0.02mg | £150.00 |
Quantity:
PSI-3289-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Images
Documents
Further Information
Additional Names:
MD-2 Antibody: MD2, MD-2, ly-96, ESOP-1, ESOP1, MD2, Lymphocyte antigen 96, Ly-96
Application Note:
WB: 0.5-1 μg/mL; IHC: 2-2.5 μg/mL; IF: 10-20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human and rat samples; Immunofluorescence in human and rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human and rat samples; Immunofluorescence in human and rat samples. All other applications and species not yet tested.
Background:
MD-2 Antibody: MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.
Background References:
- O'Neill et al. Trends in Imm. 2003; 24:286-9.
- Vogel. Mol. Interv. 2003; 3:466-77.
- Takeda et al. Annu. Rev. Immunol. 2003; 21:335-76.
- Janeway et al. Annu. Rev. Immunol. 2002; 20:197-216.
Buffer:
MD-2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Anti-MD-2 antibody (3289) was raised against a peptide corresponding to 13 amino acids near the center of human MD-2.
The immunogen is located within the last 50 amino acids of MD-2.
The immunogen is located within the last 50 amino acids of MD-2.
ISOFORMS:
Human MD-2 has 2 isoforms, including isoform 1 (160aa, 18kD) and isoform 2 (130aa, 15kD). Mouse MD-2 has one isoform (160aa, 18kD) and Rat MD-2 also has one isoform (160aa, 18kD). 3289 can detect human, mouse and rat.
NCBI Gene ID #:
23643
NCBI Official Name:
lymphocyte antigen 96
NCBI Official Symbol:
LY96
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 19kD
Observed: 26 kD (Post-modification: 2 N-linked glycosylations)
Observed: 26 kD (Post-modification: 2 N-linked glycosylations)
Protein Accession #:
NP_056179
Protein GI Number:
223555998
Purification:
MD-2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
Swissprot #:
Q9Y6Y9
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Recombinant Protein Test (Figure 1): Anti-MD-2 antibodies (3289) detected human MD-2 recombinant protein at different concentrations.
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