DAD1 Antibody
| Code | Size | Price |
|---|
| PSI-3313-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-3313-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
DAD1 Antibody: OST2, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1, Defender against cell death 1, Oligosaccharyl transferase subunit DAD1
Application Note:
DAD1 antibody can be used for detection of DAD1 by Western blot at 0.5 to 2 μg/mL. Despite its predicted molecular weight, DAD1 migrates at ~22 kDa in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
DAD1 Antibody: Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death, a process important for normal organism development and tissue homeostasis. DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity. Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death. Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas, indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.
Background References:
- Nakashima T, Sekiguchi T, Kuraoka A, et al. Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells. Mol. Cell Biol. 1993; 13:6367-74.
- Stellar H. Mechanisms and genes of cellular suicide. Science 1995; 267:1445-9.
- Gilmore R and Kelleher DJ. DAD1, the defender against apoptotic cell death, is a subunit of the mammalian oligosaccharyltransferase. Proc. Natl. Acad. Sci. USA 1997; 94:4994-9.
- Sanjay A, Fu J, and Kreibich G. DAD1 is required for the function and the structural integrity of the oligosaccharyltransferase complex. J. Biol. Chem. 1998; 273:26094-9.
Buffer:
DAD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%), Pig: (100%), Bovine: (100%), Chicken: (85%)
Immunogen:
DAD1 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human DAD1.
The immunogen is located within the last 50 amino acids of DAD1.
The immunogen is located within the last 50 amino acids of DAD1.
NCBI Gene ID #:
1603
NCBI Official Name:
defender against cell death 1
NCBI Official Symbol:
DAD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAH09798
Protein GI Number:
14602573
Purification:
DAD1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
Swissprot #:
P61803
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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